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Literature summary for 3.2.1.105 extracted from

  • Luijendijk, T.J.C.; Nowak, A.; Verpoorte, R.
    Strictosidine glucosidase from suspension cultured cells of Tabernaemontana divaricata (1996), Phytochemistry, 41, 1451-1456.
No PubMed abstract available
Search for more literature for 3.2.1.105

General Stability

General Stability Organism
highly increased sensitivity for freezing and thawing after separation over Superose 6 Tabernaemontana divaricata

Inhibitors

Inhibitors Comment Organism Structure
D(+)-gluconic acid delta-lactone some inhibition at a concentration of 10 mM and higher Tabernaemontana divaricata

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.25 0.33 strictosidine enzyme form SGIII Tabernaemontana divaricata
1
-
 strictosidine above, enzyme form SGII Tabernaemontana divaricata

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
22000
-
 enzyme form SGII, non-denaturing PAGE Tabernaemontana divaricata
31000
-
 enzyme form SGIII, non-denaturing PAGE Tabernaemontana divaricata
187000
-
 enzyme form SGII, non-denaturing PAGE Tabernaemontana divaricata
190000
-
 enzyme form SGII, non-denaturing PAGE Tabernaemontana divaricata
670000
-
 above, enzyme form SGI, gel filtration Tabernaemontana divaricata

Organism

Organism UniProt Comment Textmining
Tabernaemontana divaricata
-
 cell line 59 and cell line 6B
-

Purification (Commentary)

Purification (Comment) Organism
partial, three enzyme forms: SGI, SGII and SGIII Tabernaemontana divaricata

Source Tissue

Source Tissue Comment Organism Textmining
cell suspension culture
-
 Tabernaemontana divaricata
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Tabernaemontana divaricata

Storage Stability

Storage Stability Organism
-80°C, stable for 6 months Tabernaemontana divaricata
4°C, 24 h, stable Tabernaemontana divaricata

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no activity with secologanin, strictosamide or p-nitrophenyl-beta-D-glucopyranoside Tabernaemontana divaricata ?
-
 ?
strictosidine + H2O
-
 Tabernaemontana divaricata D-glucose + strictosidine aglycone the major intermediate after rearrangement of the strictosidine aglycone is thought to be 21-hydroxyajmalicine ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5 5 enzyme form SGI, a second pH-optimum at pH 7.0 Tabernaemontana divaricata
7
-
 enzyme form SGI, a second pH-optimum at pH 4.5-5.0 Tabernaemontana divaricata