General Stability | Organism |
---|---|
Ca2+ stabilizes | Bacteroides fragilis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 1 mM, partial inhibition | Bacteroides fragilis | |
Fe2+ | 1 mM, partial inhibition | Bacteroides fragilis | |
Galbeta1-4GlcNAcbeta1-3Gal | when assayed with lacto-N-neotetraosylceramide | Bacteroides fragilis | |
GlcNAcbeta1-3Gal | when assayed with lacto-N-neotetraosylceramide | Bacteroides fragilis | |
PCMB | 1 mM, complete inhibition | Bacteroides fragilis | |
Zn2+ | 1 mM, partial inhibition | Bacteroides fragilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
gel filtration | Bacteroides fragilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacteroides fragilis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Bacteroides fragilis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
156 | - |
- |
Bacteroides fragilis |
Storage Stability | Organism |
---|---|
-20°C, 0.044 mg/ml protein, stable for at least 12 months | Bacteroides fragilis |
4°C, stable for at least 2-3 months at a protein concentration of 0.044 mg/ml protein, 20% loss of activity in 3 h at a protein concentration of 0.003 mg/ml | Bacteroides fragilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Galbeta1-3GlcNAcbeta1-3Galbeta1-4Glc + H2O | - |
Bacteroides fragilis | ? | - |
? | |
Galbeta1-3[Fucalpha1-4]GlcNAcbeta1-3Galbeta1-4Glc + H2O | - |
Bacteroides fragilis | ? | - |
? | |
Galbeta1-4GlcNAcbeta1-3Galbeta1-4Glc + H2O | - |
Bacteroides fragilis | ? | - |
? | |
keratan sulfate + H2O | from bovine cornea | Bacteroides fragilis | keratan oligosaccharides | - |
? | |
lacto-N-neotetraosylceramide + H2O | - |
Bacteroides fragilis | ? | - |
? | |
additional information | hydrolyzes the type 2 sequence Galbeta1-4GlcNAcbeta1-3Galbeta1-4Glc at about twice the rate of the type 1 isomer, Galbeta1-3GlcNAcbeta1-3Galbeta1-4Glc | Bacteroides fragilis | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Bacteroides fragilis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
0.003 mg/ml protein, 40% loss of activity after 30 min | Bacteroides fragilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.7 | - |
- |
Bacteroides fragilis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3.5 | 9 | 25°C, 16 h, stable in presence of 0.2 mg of albumin per ml | Bacteroides fragilis |