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Literature summary for 3.2.1.103 extracted from
- Purification and characterization of endo-beta-galactosidase from Escherichia freundii induced by hog gastric mucin (1981), J. Biol. Chem., 256, 3900-3905.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.3 | - |
paragloboside | - |
Citrobacter freundii |  |
| 0.35 | - |
Keratan sulfate | - |
Citrobacter freundii | |
| 3.91 | - |
lacto-N-tetraose | - |
Citrobacter freundii | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Citrobacter freundii | induced with high efficiency in culture medium containing Smith-degraded gastric mucin | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Citrobacter freundii | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Citrobacter freundii |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 62.8 | - |
- |
Citrobacter freundii |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, stable for several months | Citrobacter freundii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| A1-glycolipid + H2O | - |
Citrobacter freundii | ? | - |
? | |
| Ab-glycolipid + H2O | - |
Citrobacter freundii | ? | - |
? | |
| H1-glycolipid + H2O | - |
Citrobacter freundii | ? | - |
? | |
| keratan sulfate + H2O | - |
Citrobacter freundii | keratan oligosaccharides | - |
? | |
| lacto-N-fucopentaose I + H2O | - |
Citrobacter freundii | ? | - |
? | |
| lacto-N-fucopentaose II + H2O | - |
Citrobacter freundii | ? | - |
? | |
| lacto-N-fucopentaose III + H2O | - |
Citrobacter freundii | ? | - |
? | |
| lacto-N-tetraose + H2O | - |
Citrobacter freundii | glucose + Galbeta(1-3)GlcNAcbeta(1-3)Gal | - |
? | |
| lacto-N-tetraose + H2O | - |
Citrobacter freundii | ? | - |
? | |
| additional information | induced with high efficiency in culture medium containing Smith-degraded gastric mucin | Citrobacter freundii | ? | - |
? | |
| paragloboside + H2O | - |
Citrobacter freundii | ? | - |
? | |
| sialosylparagloboside + H2O | - |
Citrobacter freundii | ? | - |
? | |
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