KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.065 | - |
4-nitrophenyl alpha-D-maltoheptaoside-4,6-O-ethylidene | pH not specified in the publication, 25°C | Sus scrofa | |
0.13 | - |
4-nitrophenyl alpha-D-maltoheptaoside-4,6-O-ethylidene | pH not specified in the publication, 25°C | Drosophila melanogaster | |
0.223 | - |
4-nitrophenyl alpha-D-maltoheptaoside-4,6-O-ethylidene | pH not specified in the publication, 25°C | Pseudoalteromonas haloplanktis | |
0.26 | - |
4-nitrophenyl alpha-D-maltoheptaoside-4,6-O-ethylidene | pH not specified in the publication, 25°C | Thermobifida fusca |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Drosophila melanogaster | P08144 | isoform Amy-p | - |
Pseudoalteromonas haloplanktis | P29957 | - |
- |
Sus scrofa | P00690 | isoform Amy2 | - |
Thermobifida fusca | Q47R94 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
pancreas | - |
Sus scrofa | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl alpha-D-maltoheptaoside-4,6-O-ethylidene + H2O | - |
Sus scrofa | 4-nitrophenol + 4,6-O-ethylidene-[G7]-alpha-D-maltoheptaose | - |
? | |
4-nitrophenyl alpha-D-maltoheptaoside-4,6-O-ethylidene + H2O | - |
Thermobifida fusca | 4-nitrophenol + 4,6-O-ethylidene-[G7]-alpha-D-maltoheptaose | - |
? | |
4-nitrophenyl alpha-D-maltoheptaoside-4,6-O-ethylidene + H2O | - |
Pseudoalteromonas haloplanktis | 4-nitrophenol + 4,6-O-ethylidene-[G7]-alpha-D-maltoheptaose | - |
? | |
4-nitrophenyl alpha-D-maltoheptaoside-4,6-O-ethylidene + H2O | - |
Drosophila melanogaster | 4-nitrophenol + 4,6-O-ethylidene-[G7]-alpha-D-maltoheptaose | - |
? | |
starch + H2O | - |
Sus scrofa | ? | - |
? | |
starch + H2O | - |
Thermobifida fusca | ? | - |
? | |
starch + H2O | - |
Pseudoalteromonas haloplanktis | ? | - |
? | |
starch + H2O | - |
Drosophila melanogaster | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
29 | - |
- |
Pseudoalteromonas haloplanktis |
54 | - |
- |
Sus scrofa |
54 | - |
- |
Drosophila melanogaster |
72 | - |
- |
Thermobifida fusca |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
44.1 | - |
melting temperature | Pseudoalteromonas haloplanktis |
57.7 | - |
melting temperature | Drosophila melanogaster |
65 | - |
melting temperature | Sus scrofa |
74 | - |
melting temperature | Thermobifida fusca |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
153 | - |
4-nitrophenyl alpha-D-maltoheptaoside-4,6-O-ethylidene | pH not specified in the publication, 25°C | Thermobifida fusca | |
179 | - |
starch | pH not specified in the publication, 5°C | Pseudoalteromonas haloplanktis | |
280 | - |
starch | pH not specified in the publication, 20°C | Drosophila melanogaster | |
291 | - |
4-nitrophenyl alpha-D-maltoheptaoside-4,6-O-ethylidene | pH not specified in the publication, 25°C | Sus scrofa | |
364 | - |
4-nitrophenyl alpha-D-maltoheptaoside-4,6-O-ethylidene | pH not specified in the publication, 25°C | Drosophila melanogaster | |
518 | - |
starch | pH not specified in the publication, 37°C | Sus scrofa | |
675 | - |
4-nitrophenyl alpha-D-maltoheptaoside-4,6-O-ethylidene | pH not specified in the publication, 25°C | Pseudoalteromonas haloplanktis | |
1457 | - |
starch | pH not specified in the publication, 55°C | Thermobifida fusca |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | 7.5 | - |
Sus scrofa |
7.2 | 7.5 | - |
Thermobifida fusca |
7.2 | 7.5 | - |
Pseudoalteromonas haloplanktis |
7.2 | 7.5 | - |
Drosophila melanogaster |
General Information | Comment | Organism |
---|---|---|
physiological function | comparison of chloride-dependent alpha-amylases from a psychrophilic Antarctic bacterium, the ectothermic fruit fly, the homeothermic pig and from a thermophilic actinomycete reveals striking continuum in the functional properties of the enzymes coupled to their structural stability and related to the thermal regime of the source organism | Sus scrofa |
physiological function | comparison of chloride-dependent alpha-amylases from a psychrophilic Antarctic bacterium, the ectothermic fruit fly, the homeothermic pig and from a thermophilic actinomycete reveals striking continuum in the functional properties of the enzymes coupled to their structural stability and related to the thermal regime of the source organism | Thermobifida fusca |
physiological function | comparison of chloride-dependent alpha-amylases from a psychrophilic Antarctic bacterium, the ectothermic fruit fly, the homeothermic pig and from a thermophilic actinomycete reveals striking continuum in the functional properties of the enzymes coupled to their structural stability and related to the thermal regime of the source organism | Pseudoalteromonas haloplanktis |
physiological function | comparison of chloride-dependent alpha-amylases from a psychrophilic Antarctic bacterium, the ectothermic fruit fly, the homeothermic pig and from a thermophilic actinomycete reveals striking continuum in the functional properties of the enzymes coupled to their structural stability and related to the thermal regime of the source organism | Drosophila melanogaster |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
600 | - |
4-nitrophenyl alpha-D-maltoheptaoside-4,6-O-ethylidene | pH not specified in the publication, 25°C | Thermobifida fusca | |
2800 | - |
4-nitrophenyl alpha-D-maltoheptaoside-4,6-O-ethylidene | pH not specified in the publication, 25°C | Drosophila melanogaster | |
3000 | - |
4-nitrophenyl alpha-D-maltoheptaoside-4,6-O-ethylidene | pH not specified in the publication, 25°C | Pseudoalteromonas haloplanktis | |
4500 | - |
4-nitrophenyl alpha-D-maltoheptaoside-4,6-O-ethylidene | pH not specified in the publication, 25°C | Sus scrofa |