Cloned (Comment) | Organism |
---|---|
expression as inclusion bodies in Escherichia coli | Pyrococcus furiosus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Pyrococcus furiosus | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus furiosus | O08452 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme expressed as inclusion bodies in Escherichia coli. The solubilization of the inclusion bodies is achieved by 90°C treatment for 3 min in Britton-Robinson buffer (0.04 M H3BO3, 0.04 M H3PO4, 0.04 M CH3COOH) at pH 10.5. The solubilized PFA is then diluted and subsequently purified by Phenyl Sepharose chromatography | Pyrococcus furiosus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3644 | - |
- |
Pyrococcus furiosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
soluble starch + H2O | - |
Pyrococcus furiosus | ? | - |
? |