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Literature summary for 3.2.1.1 extracted from

  • Wang, L.; Zhou, Q.; Chen, H.; Chu, Z.; Lu, J.; Zhang, Y.; Yang, S.
    Efficient solubilization, purification of recombinant extracellular alpha-amylase from Pyrococcus furiosus expressed as inclusion bodies in Escherichia coli (2007), J. Ind. Microbiol. Biotechnol., 34, 187-192.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression as inclusion bodies in Escherichia coli Pyrococcus furiosus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Pyrococcus furiosus
-
-

Organism

Organism UniProt Comment Textmining
Pyrococcus furiosus O08452
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme expressed as inclusion bodies in Escherichia coli. The solubilization of the inclusion bodies is achieved by 90°C treatment for 3 min in Britton-Robinson buffer (0.04 M H3BO3, 0.04 M H3PO4, 0.04 M CH3COOH) at pH 10.5. The solubilized PFA is then diluted and subsequently purified by Phenyl Sepharose chromatography Pyrococcus furiosus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3644
-
-
Pyrococcus furiosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
soluble starch + H2O
-
Pyrococcus furiosus ?
-
?