Protein Variants | Comment | Organism |
---|---|---|
additional information | hybrid Bacillus amyloliquefaciens X Bacillus licheniformis alpha-amylase, expression in Escherichia coli and Bacillus subtilis. The letters A and L in the hybrid names stand for the Bacillus amyloliquefaciens and the Bacillus licheniformis portion, respectively, and the numbers for the amino acid residues at the cross-over sites of the hybrid enzymes: Al76, Al108, AL112, AL142, AL147, AL149, AL151, LAL19-153, AL163, AL174 | Bacillus licheniformis |
additional information | hybrid Bacillus amyloliquefaciens X Bacillus licheniformis alpha-amylase, expression in Escherichia coli and Bacillus subtilis. The letters A and L in the hybrid names stand for the Bacillus amyloliquefaciens and the Bacillus licheniformis portion, respectively, and the numbers for the amino acid residues at the cross-over sites of the hybrid enzymes: Al76, Al108, AL112, AL142, AL147, AL149, AL151, LAL19-153, AL163, AL174 | Bacillus amyloliquefaciens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus amyloliquefaciens | - |
hybrid Bacillus amyloliquefaciens X Bacillus licheniformis alpha-amylase | - |
Bacillus licheniformis | - |
hybrid Bacillus amyloliquefaciens X Bacillus licheniformis alpha-amylase | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
p-nitrophenyl-alpha-D-maltopentaoside + H2O | - |
Bacillus licheniformis | ? | - |
? | |
p-nitrophenyl-alpha-D-maltopentaoside + H2O | poor substrate | Bacillus amyloliquefaciens | ? | - |
? | |
p-nitrophenyl-alpha-D-maltopentaoside + H2O | good substrate | Bacillus amyloliquefaciens | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
at pH 7.6 | Bacillus amyloliquefaciens |
45 | - |
at pH 6.4 | Bacillus amyloliquefaciens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
78 | - |
half-life of the mosaic hybrid enzymes ALA76-151, ALA17-151: 5 min | Bacillus licheniformis |
78 | - |
half-life of the mosaic hybrid enzymes ALA76-151, ALA17-151: 5 min | Bacillus amyloliquefaciens |
90 | - |
half-life: 5 min | Bacillus amyloliquefaciens |
90 | - |
30 min, 10-20% loss of activity of the hybrid enzymes AL76, AL108, and AL112, 35-50% loss of activity of the hybrid enzymes AL142, AL147, AL149, AL151, LAL19-163, AL163, and AL174. About 90% of activity after 30 min of the hybrid enzymes AL231, AL256 and AL263 | Bacillus licheniformis |
90 | - |
30 min, 10-20% loss of activity of the hybrid enzymes AL76, AL108, and AL112, 35-50% loss of activity of the hybrid enzymes AL142, AL147, AL149, AL151, LAL19-163, AL163, and AL174. About 90% of activity after 30 min of the hybrid enzymes AL231, AL256 and AL263 | Bacillus amyloliquefaciens |
90 | - |
half-life of hybrid enzymes: about 50 min for LA431, AL17, Al34 | Bacillus licheniformis |
90 | - |
half-life of hybrid enzymes: about 50 min for LA431, AL17, Al34 | Bacillus amyloliquefaciens |
90 | - |
half-life: 52 min | Bacillus licheniformis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.4 | 7.6 | broad | Bacillus licheniformis |
6.4 | 7.6 | broad | Bacillus amyloliquefaciens |