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Literature summary for 3.13.2.3 extracted from

  • Deng, H.; McMahon, S.; Eustáquio, A.; Moore, B.; Naismith, J.; OHagan, D.
    Mechanistic insights into water activation in SAM hydroxide adenosyltransferase (duf-62) (2009), ChemBioChem, 10, 2455-2459 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.13.2.3

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Salinispora arenicola
expressed in Escherichia coli Rosetta cells Pyrococcus horikoshii

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method, using PEG 6000 (19.83 %), KCl (0.26 M) and MOPS (0.1 M, pH 7.0) Pyrococcus horikoshii
sitting drop vapor diffusion method, using PEG 6000 (19.83 %), KCl (0.26 M) and MOPS (0.1 M, pH 7.0) Salinispora arenicola

Protein Variants

Protein Variants Comment Organism
D72A inactive Salinispora arenicola
H130A the mutant shows about 25% of wild type activity Salinispora arenicola
H130Y the mutant shows about 5% of wild type activity Salinispora arenicola
R79A the mutant shows about 0.15% of wild type activity Salinispora arenicola

Inhibitors

Inhibitors Comment Organism Structure
S-adenosyl-L-homocysteine
-
 Pyrococcus horikoshii
S-adenosyl-L-homocysteine
-
 Salinispora arenicola

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.001
-
 S-adenosyl-L-methionine wild type enzyme, at pH 7.9 and 37°C Salinispora arenicola
0.0014
-
 S-adenosyl-L-methionine mutant enzyme H130A, at pH 7.9 and 37°C Salinispora arenicola
0.0017
-
 S-adenosyl-L-methionine mutant enzyme H130Y, at pH 7.9 and 37°C Salinispora arenicola

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + H2O Pyrococcus horikoshii
-
 adenosine + L-methionine
-
 ?
S-adenosyl-L-methionine + H2O Salinispora arenicola
-
 adenosine + L-methionine
-
 ?

Organism

Organism UniProt Comment Textmining
Pyrococcus horikoshii O58212
-
-
Salinispora arenicola
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pyrococcus horikoshii
-
 Salinispora arenicola

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + H2O
-
 Pyrococcus horikoshii adenosine + L-methionine
-
 ?
S-adenosyl-L-methionine + H2O
-
 Salinispora arenicola adenosine + L-methionine
-
 ?

Synonyms

Synonyms Comment Organism
duf-62
-
 Pyrococcus horikoshii
duf-62
-
 Salinispora arenicola
S-adenosyl-L-methionine hydrolase
-
 Pyrococcus horikoshii
S-adenosyl-L-methionine hydrolase
-
 Salinispora arenicola
SAM hydroxide adenosyltransferase
-
 Pyrococcus horikoshii
SAM hydroxide adenosyltransferase
-
 Salinispora arenicola

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00066
-
 S-adenosyl-L-methionine mutant enzyme H130Y, at pH 7.9 and 37°C Salinispora arenicola
0.0027
-
 S-adenosyl-L-methionine mutant enzyme H130A, at pH 7.9 and 37°C Salinispora arenicola
0.0075
-
 S-adenosyl-L-methionine wild type enzyme, at pH 7.9 and 37°C Salinispora arenicola

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.38
-
 S-adenosyl-L-methionine mutant enzyme H130Y, at pH 7.9 and 37°C Salinispora arenicola
1.9
-
 S-adenosyl-L-methionine mutant enzyme H130A, at pH 7.9 and 37°C Salinispora arenicola
7.5
-
 S-adenosyl-L-methionine wild type enzyme, at pH 7.9 and 37°C Salinispora arenicola