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Literature summary for 3.13.2.3 extracted from

  • Deng, H.; Botting, C.; Hamilton, J.; Russell, R.; OHagan, D.
    S-adenosyl-L-methionine Hydroxide adenosyltransferase A SAM enzyme (2008), Angew. Chem. Int. Ed. Engl., 47, 5357-5361 .
No PubMed abstract available
Search for more literature for 3.13.2.3

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Pyrococcus horikoshii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0392
-
 S-adenosyl-L-methionine at pH 8.5 and 37°C Pyrococcus horikoshii
0.41
-
 5'-chloro-5'-deoxyadenosine at pH 8.5 and 37°C Pyrococcus horikoshii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + H2O Pyrococcus horikoshii
-
 adenosine + L-methionine + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Pyrococcus horikoshii O58212
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5'-chloro-5'-deoxyadenosine + H2O low activity Pyrococcus horikoshii ?
-
 ?
additional information no activity with 5'-fluoro-5'-deoxyadenosine Pyrococcus horikoshii ?
-
 ?
S-adenosyl-L-methionine + H2O
-
 Pyrococcus horikoshii adenosine + L-methionine + H+
-
 ?

Synonyms

Synonyms Comment Organism
duf62
-
 Pyrococcus horikoshii
SAM hydroxide adenosyltransferase
-
 Pyrococcus horikoshii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Pyrococcus horikoshii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
 the enzyme retains full activity after being heated at 80°C for 30 min and then cooled to 37°C Pyrococcus horikoshii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0052
-
 5'-chloro-5'-deoxyadenosine at pH 8.5 and 37°C Pyrococcus horikoshii
0.14
-
 S-adenosyl-L-methionine at pH 8.5 and 37°C Pyrococcus horikoshii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
 Pyrococcus horikoshii

pH Range

pH Minimum pH Maximum Comment Organism
5 10 the enzyme is inactive below pH 5.0 and above pH 10.0 Pyrococcus horikoshii