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Literature summary for 3.13.1.3 extracted from

  • Nakayama, N.; Matsubara, T.; Ohshiro, T.; Moroto, Y.; Kawata, Y.; Koizumi, K.; Hirakawa, Y.; Suzuki, M.; Maruhashi, K.; Izumi, Y.; Kurane, R.
    A novel enzyme, 2'-hydroxybiphenyl-2-sulfinate desulfinase (DszB), from a dibenzothiophene-desulfurizing bacterium Rhodococcus erythropolis KA2-5-1: gene overexpression and enzyme characterization (2002), Biochim. Biophys. Acta, 1598, 122-130.
    View publication on PubMed
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Activating Compound

Activating Compound Comment Organism Structure
additional information no activation by metal ions Rhodococcus erythropolis

Cloned(Commentary)

Cloned (Comment) Organism
gene dszZ, DNA sequence determination, cooverexpression in Escherichia coli with chaperonin genes groEL and groES at 25°C Rhodococcus erythropolis

Protein Variants

Protein Variants Comment Organism
C26S site-directed mutagenesis of the only cysteine residue results in an completely inactive mutant Rhodococcus erythropolis

Inhibitors

Inhibitors Comment Organism Structure
2,2'-Dihydroxybiphenyl competitive Rhodococcus erythropolis
2,3-Dihydroxybiphenyl competitive Rhodococcus erythropolis
2-Hydroxybiphenyl competitive, product inhibition Rhodococcus erythropolis
Ag+ strong inhibition at 1 mM Rhodococcus erythropolis
Cu2+ strong inhibition at 1 mM Rhodococcus erythropolis
DTNB strong inhibition at 1 mM Rhodococcus erythropolis
Hg2+ strong inhibition at 1 mM Rhodococcus erythropolis
additional information no inhibition by product sulfite, no inhibition by chelating agents EDTA, 1,10-phenanthroline, and 8-hydroxyquinone Rhodococcus erythropolis
N-ethylmaleimide strong inhibition at 1 mM Rhodococcus erythropolis
p-chloromercuribenzoate strong inhibition at 1 mM Rhodococcus erythropolis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0082
-
 2'-hydroxybiphenyl-2-sulfinate pH 7.0, 28°C Rhodococcus erythropolis
0.062
-
 2-phenylbenzenesulfinate pH 7.0, 28°C Rhodococcus erythropolis

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no metal ions required Rhodococcus erythropolis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
 1 * 40000, SDS-PAGE Rhodococcus erythropolis
43000
-
 gel filtration Rhodococcus erythropolis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2'-hydroxybiphenyl-2-sulfinate + H2O Rhodococcus erythropolis
-
 2-hydroxybiphenyl + sulfite
-
 ?
2'-hydroxybiphenyl-2-sulfinate + H2O Rhodococcus erythropolis KA2-5-1
-
 2-hydroxybiphenyl + sulfite
-
 ?

Organism

Organism UniProt Comment Textmining
Rhodococcus erythropolis
-
-
-
Rhodococcus erythropolis KA2-5-1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant 7.8fold from Escherichia coli, and native from strain KA2-5-1 160fold, to homogeneity Rhodococcus erythropolis

Reaction

Reaction Comment Organism Reaction ID
2'-hydroxybiphenyl-2-sulfinate + H2O = 2-hydroxybiphenyl + sulfite Cys26 is essential for activity Rhodococcus erythropolis
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.18
-
 purified native enzyme Rhodococcus erythropolis
0.207
-
 purified recombinant enzyme Rhodococcus erythropolis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2'-hydroxybiphenyl-2-sulfinate + H2O
-
 Rhodococcus erythropolis 2-hydroxybiphenyl + sulfite
-
 ?
2'-hydroxybiphenyl-2-sulfinate + H2O highly specific for Rhodococcus erythropolis 2-hydroxybiphenyl + sulfite
-
 ?
2'-hydroxybiphenyl-2-sulfinate + H2O
-
 Rhodococcus erythropolis KA2-5-1 2-hydroxybiphenyl + sulfite
-
 ?
2'-hydroxybiphenyl-2-sulfinate + H2O highly specific for Rhodococcus erythropolis KA2-5-1 2-hydroxybiphenyl + sulfite
-
 ?
2-phenylbenzenesulfinate + H2O
-
 Rhodococcus erythropolis biphenyl + sulfite
-
 ?
2-phenylbenzenesulfinate + H2O
-
 Rhodococcus erythropolis KA2-5-1 biphenyl + sulfite
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 40000, SDS-PAGE Rhodococcus erythropolis

Synonyms

Synonyms Comment Organism
2'-hydroxybiphenyl-2-sulfinate desulfinase
-
 Rhodococcus erythropolis
DszB
-
 Rhodococcus erythropolis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
 recombinant enzyme Rhodococcus erythropolis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
28
-
 recombinant enzyme, stable Rhodococcus erythropolis
30
-
 recombinant enzyme, rapid loss of activity above, inactivation after 4 h Rhodococcus erythropolis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 about, recombinant enzyme Rhodococcus erythropolis

Cofactor

Cofactor Comment Organism Structure
additional information no cofactor required Rhodococcus erythropolis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.25
-
 2-Hydroxybiphenyl pH 7.0, 28°C, recombinant enzyme Rhodococcus erythropolis
0.4
-
 2,3-Dihydroxybiphenyl pH 7.0, 28°C, recombinant enzyme Rhodococcus erythropolis
2
-
 2,2'-Dihydroxybiphenyl pH 7.0, 28°C, recombinant enzyme Rhodococcus erythropolis