Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | 1.23fold activation at 1 mM | Paenibacillus sp. |
Cloned (Comment) | Organism |
---|---|
gene tdsB, DNA sequence determination, overexpression in Escherichia coli BL21 (DE3) | Paenibacillus sp. |
Protein Variants | Comment | Organism |
---|---|---|
C17S | site-directed mutagenesis of the only cysteine residue results in an completely inactive mutant | Paenibacillus sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-Hydroxybiphenyl | 30% inhibition at 5 mM, the OH-group is important for the inhibitory effect | Paenibacillus sp. | |
Cu2+ | 67% inhibition at 1 mM | Paenibacillus sp. | |
DTNB | 86% inhibition at 0.1 mM | Paenibacillus sp. | |
Fe2+ | 44% inhibition at 1 mM | Paenibacillus sp. | |
iodoacetic acid | 95% inhibition at 1 mM | Paenibacillus sp. | |
Mn2+ | 71% inhibition at 1 mM | Paenibacillus sp. | |
additional information | no inhibition by 1,10-phenanthroline and EDTA, no inhibition by Co2+, Ca2+, and Mg2+, no inhibition by biphenyl, 2-methylbiphenyl, and 2-methoxybiphenyl | Paenibacillus sp. | |
N-ethylmaleimide | complete inhibition at 1 mM | Paenibacillus sp. | |
Ni2+ | 24% inhibition at 1 mM | Paenibacillus sp. | |
o-hydroxystyrene | product inhibition, 80% inhibition at 5 mM, the OH-group is important for the inhibitory effect | Paenibacillus sp. | |
p-chloromercuribenzoate | 92% inhibition at 0.1 mM | Paenibacillus sp. | |
Zn2+ | 98% inhibition at 1 mM | Paenibacillus sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.33 | - |
2-(2-hydroxyphenyl)benzenesulfinate | pH 7.0, 50°C | Paenibacillus sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
31000 | - |
gel filtration | Paenibacillus sp. |
39000 | - |
1 * 39000, SDS-PAGE | Paenibacillus sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-(2'-hydroxyphenyl)benzenesulfinate + H2O | Paenibacillus sp. | final step of the desulfurozation of dibenzothiophene | 2-hydroxybiphenyl + sulfite | - |
ir | |
2-(2'-hydroxyphenyl)benzenesulfinate + H2O | Paenibacillus sp. A11-2 | final step of the desulfurozation of dibenzothiophene | 2-hydroxybiphenyl + sulfite | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Paenibacillus sp. | - |
- |
- |
Paenibacillus sp. A11-2 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme from strain A11-2 63fold | Paenibacillus sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.139 | - |
purified enzyme | Paenibacillus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-(2'-hydroxyphenyl)benzenesulfinate + H2O | - |
Paenibacillus sp. | 2-hydroxybiphenyl + sulfite | - |
ir | |
2-(2'-hydroxyphenyl)benzenesulfinate + H2O | final step of the desulfurozation of dibenzothiophene | Paenibacillus sp. | 2-hydroxybiphenyl + sulfite | - |
ir | |
2-(2'-hydroxyphenyl)benzenesulfinate + H2O | - |
Paenibacillus sp. A11-2 | 2-hydroxybiphenyl + sulfite | - |
ir | |
2-(2'-hydroxyphenyl)benzenesulfinate + H2O | final step of the desulfurozation of dibenzothiophene | Paenibacillus sp. A11-2 | 2-hydroxybiphenyl + sulfite | - |
ir | |
2-(2'-hydroxyphenyl)ethene-1-sulfinate + H2O | - |
Paenibacillus sp. | o-hydroxystyrene + sulfite | - |
? | |
2-(2'-hydroxyphenyl)ethene-1-sulfinate + H2O | - |
Paenibacillus sp. A11-2 | o-hydroxystyrene + sulfite | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 39000, SDS-PAGE | Paenibacillus sp. |
Synonyms | Comment | Organism |
---|---|---|
2-(2'-hydroxyphenyl)benzene sulfinate desulfinase | - |
Paenibacillus sp. |
TdsB | - |
Paenibacillus sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Paenibacillus sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
30 min, stable up to | Paenibacillus sp. |
60 | - |
rapid loss of activity | Paenibacillus sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Paenibacillus sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | no cofactors required | Paenibacillus sp. |