Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | - |
Arabidopsis thaliana | 9507 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-glucose + ? | Arabidopsis thaliana | first step of sulfolipid biosynthesis, unknown sulfur donor | UDP-sulfoquinovose + H2O | - |
? | |
UDP-glucose + sulfite | Arabidopsis thaliana | - |
UDP-sulfoquinovose + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni2+-nitrilotriacetic acid column | Arabidopsis thaliana |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
UDP-alpha-D-sulfoquinovopyranose + H2O = UDP-alpha-D-glucose + sulfite | sequence similarity to sugar nucleotide modifying enzymes, Thr228 seems to be catalytically important | Arabidopsis thaliana | |
UDP-alpha-D-sulfoquinovopyranose + H2O = UDP-alpha-D-glucose + sulfite | short-chain dehydrogenase/reductase family | Arabidopsis thaliana |
Storage Stability | Organism |
---|---|
4°C, 50 mM MES buffer, pH 6.5, 10 mM UDP-glucose, stable for 10 days | Arabidopsis thaliana |
4°C, 50 mM MES buffer, pH 6.5, stable for 2-3 days | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-glucose + ? | first step of sulfolipid biosynthesis, unknown sulfur donor | Arabidopsis thaliana | UDP-sulfoquinovose + H2O | - |
? | |
UDP-glucose + sulfite | - |
Arabidopsis thaliana | UDP-sulfoquinovose + H2O | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | tightly bound, enzyme binds 1 NAD+ per monomer, side-chain chemistry and hydrogen-bonding patterns in the NAD+ site | Arabidopsis thaliana |