Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | sulfate, APS, PAPS, sulfite, thiosulfate, sufide or sulfoglutathione are tested to determine whether they could stimulate the formation of UDP-sulfoquinovose | Arabidopsis thaliana |
Application | Comment | Organism |
---|---|---|
medicine | sulfolipids, in which synthesis the enzyme is involved, promise anti-tumor and anti-HIV therapeutics based on their inhibition of DNA polymerase and reverse transcriptase | Arabidopsis thaliana |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
T145A | greatly reduced activity, site-directed mutagenesis, plays critical role for catalytic activity | Arabidopsis thaliana |
T145A | mutant shows greatly reduced activity | Arabidopsis sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
sulfite | increasing concentrations beyond 0.1 mM | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
sulfite | - |
Arabidopsis thaliana | |
0.01 | - |
sulfite | - |
Arabidopsis sp. | |
0.15 | - |
UDP-glucose | - |
Arabidopsis thaliana | |
0.15 | - |
UDP-glucose | - |
Arabidopsis sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | thylakoid | Arabidopsis thaliana | 9507 | - |
membrane | photosynthetic | Arabidopsis thaliana | 16020 | - |
thylakoid | - |
Arabidopsis thaliana | 9579 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45500 | - |
recombinant protein, lacking the transit peptide | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-glucose + sulfite | Arabidopsis thaliana | sulfolipid biosynthesis | UDP-sulfoquinovose + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis sp. | - |
- |
- |
Arabidopsis thaliana | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni2+-nitrilotriacetic acid column | Arabidopsis thaliana |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0026 | - |
- |
Arabidopsis thaliana |
Storage Stability | Organism |
---|---|
-20°C, 25 mM NaH2PO4, pH 7.5, 300 mM NaCl, 20% glycerol | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-glucose + sulfite | - |
Arabidopsis thaliana | UDP-sulfoquinovose + H2O | - |
? | |
UDP-glucose + sulfite | sulfolipid biosynthesis | Arabidopsis thaliana | UDP-sulfoquinovose + H2O | - |
? | |
UDP-glucose + sulfite | - |
Arabidopsis sp. | UDP-6-sulfoquinovose + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
SQD1 | - |
Arabidopsis sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Arabidopsis thaliana |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00167 | - |
UDP-glucose | - |
Arabidopsis thaliana | |
0.1 | - |
UDP-glucose | - |
Arabidopsis sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 9.5 | - |
Arabidopsis thaliana |
9 | - |
- |
Arabidopsis sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 10 | pH 7.0: about 50% of maximal activity, pH 10.0: about 35% of maximal activity | Arabidopsis sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Arabidopsis thaliana |