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Literature summary for 3.12.1.B1 extracted from

  • Kanao, T.; Onishi, M.; Kajitani, Y.; Hashimoto, Y.; Toge, T.; Kikukawa, H.; Kamimura, K.
    Characterization of tetrathionate hydrolase from the marine acidophilic sulfur-oxidizing bacterium, Acidithiobacillus thiooxidans strain SH (2018), Biosci. Biotechnol. Biochem., 82, 152-160 .
    View publication on PubMed
Search for more literature for 3.12.1.B1

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21 StarTM(DE3) Acidithiobacillus thiooxidans

Inhibitors

Inhibitors Comment Organism Structure
KNO3 1 M, 40% loss of activity Acidithiobacillus thiooxidans
NaNO3 1 M, 20% loss of activity Acidithiobacillus thiooxidans

Localization

Localization Comment Organism GeneOntology No. Textmining
outer membrane localized on the outer membrane of the cell, and acts as a peripheral protein Acidithiobacillus thiooxidans 19867
-

Metals/Ions

Metals/Ions Comment Organism Structure
(NH4)2SO4 1 M, 4fold increase in activity Acidithiobacillus thiooxidans
KCl 2 M, 2.5fold increase in activity Acidithiobacillus thiooxidans
Na2SO4 1 M, 2.5fold increase in activity Acidithiobacillus thiooxidans
NaCl 1 M, 2.5fold increase in activity Acidithiobacillus thiooxidans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
97000
-
 gel filtration Acidithiobacillus thiooxidans

Organism

Organism UniProt Comment Textmining
Acidithiobacillus thiooxidans
-
-
-
Acidithiobacillus thiooxidans SH
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Acidithiobacillus thiooxidans

Source Tissue

Source Tissue Comment Organism Textmining
culture condition:sulfur-grown cell
-
 Acidithiobacillus thiooxidans
-
culture condition:tetrathionate-grown cell
-
 Acidithiobacillus thiooxidans
-

Subunits

Subunits Comment Organism
homodimer 2 * 52000, SDS-PAGE Acidithiobacillus thiooxidans

Synonyms

Synonyms Comment Organism
4THase
-
 Acidithiobacillus thiooxidans
SH-tth
-
 Acidithiobacillus thiooxidans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
 Acidithiobacillus thiooxidans

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 65 20°C: about 40% of maximal activity, 65°C: about 65% of maximal activity Acidithiobacillus thiooxidans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3
-
-
 Acidithiobacillus thiooxidans

pH Range

pH Minimum pH Maximum Comment Organism
1 4 pH 1.0: about 40% of maximal activity, pH 4.0: about 55% of maximal activity Acidithiobacillus thiooxidans

General Information

General Information Comment Organism
metabolism the enzyme is involved in tetrathionate metabolism, but also in thiosulfate and elemental sulfur metabolism Acidithiobacillus thiooxidans