Literature summary for 3.12.1.B1 extracted from

De Jong, G.A.H.; Hazeu, W.; Bos, P.; Kuenen, J.G.
Polythionate degradation by tetrathionate hydrolase of Thiobacillus ferrooxidans (1997), Microbiology, 143, 499-504.

Search for more literature for 3.12.1.B1

Application

Application	Comment	Organism
analysis	measurement of enzyme activity is based on a continuous spectrophotometric method that detects soluble intermediates that absorb in the UV region	Acidithiobacillus ferrooxidans

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.05	-	tetrathionate	pH 3.0, 30°C, presence of 0.5 M ammonium sulfate	Acidithiobacillus ferrooxidans

Organism

Organism	UniProt	Comment	Textmining
Acidithiobacillus ferrooxidans	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Acidithiobacillus ferrooxidans

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	no substrates: trithionate, hexathionate	Acidithiobacillus ferrooxidans	?	-	?
pentathionate + H2O	80% of the activity with tetrathionate	Acidithiobacillus ferrooxidans	2 sulfur + thiosulfate + sulfate + 2 H+	-	?
tetrathionate + H2O	the end products of hydrolysis of both tetrathionate and pentathionate by the pure enzyme are thiosulfate, sulfur and sulfate	Acidithiobacillus ferrooxidans	sulfur + thiosulfate + sulfate + 2 H+	-	?

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
65	-	-	Acidithiobacillus ferrooxidans

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
4	-	-	Acidithiobacillus ferrooxidans

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Release 2023.1 (January 2023)