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Literature summary for 3.12.1.B1 extracted from

  • Protze, J.; Mueller, F.; Lauber, K.; Nab, B.; Mentele, R.; Lottspeich, F.; Kletzin, A.
    An extracellular tetrathionate hydrolase from the thermoacidophilic archaeon Acidianus ambivalens with an activity optimum at pH 1 (2011), Front. Microbiol., 2, 68.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.12.1.B1

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.8
-
 tetrathionate pH 1, 80°C Acidianus ambivalens

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Acidianus ambivalens
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53642
-
 and dimer, 1 * 54000, SDS-PAGE, x * 53642, calculated without gylcosylation Acidianus ambivalens
54000
-
 gel filtration Acidianus ambivalens
105000
-
 gel filtration Acidianus ambivalens

Organism

Organism UniProt Comment Textmining
Acidianus ambivalens G8YXZ9
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein N-terminal amino acid sequence Gly-Pro-Ile-Val-Tyr-Thr-Tyr-Thr-Glu-Tyr-Asn-Gly-Thr-Tyr with Asn being glycosylated Acidianus ambivalens

Purification (Commentary)

Purification (Comment) Organism
partial Acidianus ambivalens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
15
-
 pH 1, 80°C Acidianus ambivalens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
tetrathionate + H2O
-
 Acidianus ambivalens sulfur + thiosulfate + sulfate + 2 H+
-
 ?

Subunits

Subunits Comment Organism
dimer and monomer, 2 * 54000, SDS-PAGE Acidianus ambivalens
monomer and dimer, 1 * 54000, SDS-PAGE, x * 53642, calculated without gylcosylation Acidianus ambivalens

Synonyms

Synonyms Comment Organism
tetrathionate hydrolase
-
 Acidianus ambivalens
Tth1
-
 Acidianus ambivalens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
95
-
-
 Acidianus ambivalens

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
additional information
-
 no activity at 40°C Acidianus ambivalens
50
-
 modest activity Acidianus ambivalens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
1
-
-
 Acidianus ambivalens

pH Range

pH Minimum pH Maximum Comment Organism
additional information
-
 no activitiy at pH 6 Acidianus ambivalens