Literature summary for 3.11.1.1 extracted from

Lahiri, S.D.; Zhang, G.; Dai, J.; Dunaway-Mariano, D.; Allen, K.N.
Analysis of the substrate specificity loop of the HAD superfamily cap domain (2004), Biochemistry, 43, 2812-2820.

Search for more literature for 3.11.1.1

Crystallization (Commentary)

Crystallization (Comment)	Organism
mutant enzymes	Bacillus cereus

Protein Variants

Protein Variants	Comment	Organism
G50A	kcat/Km is 12820fold lower than wild-type value	Bacillus cereus
G50P	inactive mutant protein	Bacillus cereus
G50V	inactive mutant protein	Bacillus cereus
H56Q	kcat/Km is 238fold lower than wild-type value	Bacillus cereus
K53A	inactive mutant protein	Bacillus cereus
K53R	inactive mutant protein	Bacillus cereus
M49L	kcat/Km is 17241fold lower than wild-type value	Bacillus cereus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.033	-	phosphonoacetaldehyde	25°C, pH 7.5, wild-type enzyme	Bacillus cereus
0.175	-	phosphonoacetaldehyde	25°C, pH 7.5, mutant enzyme H56Q	Bacillus cereus
5.1	-	phosphonoacetaldehyde	25°C, pH 7.5, mutant enzyme m49L	Bacillus cereus
11	-	phosphonoacetaldehyde	25°C, pH 7.5, mutant enzyme G50A	Bacillus cereus

Organism

Organism	UniProt	Comment	Textmining
Bacillus cereus	-	-	-

Renatured (Commentary)

Renatured (Comment)	Organism
expression of mutant enzymes in escherichia coli	Bacillus cereus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
phosphonoacetaldehyde + H2O	catalysis within the core domain of phosphonatase requires the participation of loop 5 of the corresponding cap domain. Gly is an indispensable component	Bacillus cereus	acetaldehyde + phosphate	-	?

Synonyms

Synonyms	Comment	Organism
phosphonatase	-	Bacillus cereus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.00247	-	phosphonoacetaldehyde	25°C, pH 7.5, mutant enzyme m49L	Bacillus cereus
0.006	-	phosphonoacetaldehyde	25°C, pH 7.5, mutant enzyme H56Q	Bacillus cereus
0.0071	-	phosphonoacetaldehyde	25°C, pH 7.5, mutant enzyme G50A	Bacillus cereus
0.25	-	phosphonoacetaldehyde	25°C, pH 7.5, wild-type enzyme	Bacillus cereus

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Release 2023.1 (January 2023)