Application | Comment | Organism |
---|---|---|
medicine | enzyme-replacement or gene-replacement therapy | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expression in Chinese hamster ovary cells | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0107 | - |
tetrasaccharides | substrates deriving from heparan sulfate, unamplified recombinant enzyme | Homo sapiens | |
0.01225 | - |
tetrasaccharides | substrates deriving from heparan sulfate, gene-amplified recombinant enzyme | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
54680 | - |
calculation from cDNA sequence | Homo sapiens |
57000 | - |
SDS-PAGE, mature enzyme | Homo sapiens |
62000 | - |
2 * 62000, SDS-PAGE | Homo sapiens |
63000 | - |
SDS-PAGE, precursor of recombinant enzyme | Homo sapiens |
115000 | - |
gel filtration, Superose 12 | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
heparan sulfate + H2O | Homo sapiens | - |
? + sulfate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
purification of recombinant enzyme | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
heparan sulfate + H2O | - |
Homo sapiens | ? + sulfate | - |
? | |
heparan sulfate + H2O | - |
Homo sapiens | ? + sulfate | - |
ir | |
tetrasaccharides + H2O | - |
Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 62000, SDS-PAGE | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.2 | - |
recombinant enzyme | Homo sapiens |