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Literature summary for 3.1.99.B4 extracted from
- The xthA gene product of Archaeoglobus fulgidus is an unspecific DNase (2003), Eur. J. Biochem., 270, 1838-1849.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Archaeoglobus fulgidus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | - |
Archaeoglobus fulgidus |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | weak activity when replacing Mg2+ with Ca2+ ions | Archaeoglobus fulgidus | |
| Mg2+ | weak activity when replacing Mg2+ with Ca2+ ions. Mn2+ can substitute Mg2+ at concentrations below 1 mM but results in a lower activity | Archaeoglobus fulgidus | |
| Mn2+ | Mn2+ can substitute Mg2+ at concentrations below 1 mM but results in a lower activity | Archaeoglobus fulgidus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 29760 | - |
calculated from sequence | Archaeoglobus fulgidus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Archaeoglobus fulgidus | O29675 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Archaeoglobus fulgidus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| DNA + H2O | the DNA is degraded relatively fast resulting in an end product of approximately half the length of the initial substrate. The enzyme is able to degrade supercoiled plasmids and shows no preferences for blunt or recessed 3'-termini of linear double stranded DNA. Whole DNA is digested to fragments between 100 and 500 bp with increasing incubation times | Archaeoglobus fulgidus | fragments of DNA | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| unspecific DNase | - |
Archaeoglobus fulgidus |
| xthA | - |
Archaeoglobus fulgidus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | 80 | - |
Archaeoglobus fulgidus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Archaeoglobus fulgidus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 10 | - |
Archaeoglobus fulgidus |
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Release 2023.1 (January 2023)
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