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Literature summary for 3.1.8.2 extracted from

  • Zhao, L.; Gattas-Asfura, K.M.; Xu, J.; Patel, R.A.; Dadlani, A.; Sillero-Mahinay, M.; Cushmore, M.; Rastogi, V.K.; Shah, S.S.; Leblanc, R.M.
    Organophosphorus acid anhydrolase bio-template for the synthesis of CdS quantum dots (2011), Chem. Commun. (Camb. ), 47, 7242-7244.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
additional information preparation of a direct conjugation of organophosphorus acid anhydrolase with CdS quantum dots via arrested precipitation within the enzyme matrix. The bio-conjugate not only retains enzyme conformational structure but also retains enzyme activity and is effective at detecting diisopropyl fluorophosphate at the micromolar level. Purification of the conjugates by gel filtration. Circular dichroism spectrometric and high resolution transmission electron microscope conjugate structure analysis, overview Pseudomonas aeruginosa

Metals/Ions

Metals/Ions Comment Organism Structure
Cd2+ activates Pseudomonas aeruginosa
Mn2+ binuclear Mn2+ active site Pseudomonas aeruginosa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
58500
-
x * 58500 Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
diisopropyl fluorophosphate + H2O Pseudomonas aeruginosa
-
diisopropyl phosphate + fluoride
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation
-
Pseudomonas aeruginosa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
diisopropyl fluorophosphate + H2O
-
Pseudomonas aeruginosa diisopropyl phosphate + fluoride
-
?
diisopropyl fluorophosphate + H2O binding analysis, overview Pseudomonas aeruginosa diisopropyl phosphate + fluoride
-
?

Subunits

Subunits Comment Organism
? x * 58500 Pseudomonas aeruginosa

Synonyms

Synonyms Comment Organism
OPAA
-
Pseudomonas aeruginosa
organophosphorus acid anhydrolase
-
Pseudomonas aeruginosa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
assay at Pseudomonas aeruginosa

pI Value

Organism Comment pI Value Maximum pI Value
Pseudomonas aeruginosa
-
-
7.6