Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Loligo vulgaris |
Crystallization (Comment) | Organism |
---|---|
mutant enzymes N120D/N175D/D229N, E21QN120D/N175D/D229N, and D121E are crystallized by hanging drop vapor diffusion method, using 0.1 M MES buffer pH 6.5, 14-20% (w/v) PEG 3350 | Loligo vulgaris |
Protein Variants | Comment | Organism |
---|---|---|
D121E | the mutant displays 87% activity compared to the wild type enzyme | Loligo vulgaris |
D229N | enzymatically inactive | Loligo vulgaris |
E21Q/N120D/N175D/D229N | the mutations lead to a loss of calcium binding and enzymatic activity | Loligo vulgaris |
N120D/N175D/D229N | the mutations lead to a loss of calcium binding and enzymatic activity | Loligo vulgaris |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | required for activity, the wild type enzyme contains two Ca2+ ions | Loligo vulgaris |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Loligo vulgaris | Q7SIG4 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography and Q-Sepharose column chromatography | Loligo vulgaris |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
diisopropyl fluorophosphate + H2O | - |
Loligo vulgaris | diisopropyl phosphate + fluoride | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DFPase | - |
Loligo vulgaris |
diisopropyl fluorophosphatase | - |
Loligo vulgaris |