Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Loligo vulgaris |
Crystallization (Comment) | Organism |
---|---|
wild-type and mutant N175D, 1.7 A resolution | Loligo vulgaris |
Protein Variants | Comment | Organism |
---|---|---|
D121F | no activity | Loligo vulgaris |
D229N/N120D | no activity | Loligo vulgaris |
D232S | 3% higher activity than the wild-type | Loligo vulgaris |
F173A | 84% lower activity than the wild-type | Loligo vulgaris |
F173L | 28% lower activity than the wild-type | Loligo vulgaris |
F173S | 68% lower activity than the wild-type | Loligo vulgaris |
F173V | 46% lower activity than the wild-type | Loligo vulgaris |
F173W | 19% lower activity than the wild-type | Loligo vulgaris |
F173Y | 53% lower activity than the wild-type | Loligo vulgaris |
F314A | 3% higher activity than the wild-type | Loligo vulgaris |
H181N | 19% lower activity than the wild-type | Loligo vulgaris |
H274N | 7% lower activity than the wild-type | Loligo vulgaris |
H287A | 90% lower activity than the wild-type | Loligo vulgaris |
H287D | 99% lower activity than the wild-type | Loligo vulgaris |
H287F | 36% lower activity than the wild-type | Loligo vulgaris |
H287L | 21% lower activity than the wild-type | Loligo vulgaris |
H287N | 96% lower activity than the wild-type | Loligo vulgaris |
H287Q | 54% lower activity than the wild-type | Loligo vulgaris |
H287W | 34% lower activity than the wild-type | Loligo vulgaris |
H287Y | 57% lower activity than the wild-type | Loligo vulgaris |
M148A | 26% lower activity than the wild-type | Loligo vulgaris |
N120D | 96% lower activity than the wild-type | Loligo vulgaris |
N175D | 98% lower activity than the wild-type | Loligo vulgaris |
N237S | 4% lower activity than the wild-type | Loligo vulgaris |
N272F | no activity | Loligo vulgaris |
Q304F | 50% lower activity than the wild-type | Loligo vulgaris |
Q304W | 3% lower activity than the wild-type | Loligo vulgaris |
Q77F | no activity | Loligo vulgaris |
Q77W | 6% higher activity than the wild-type | Loligo vulgaris |
Q77Y | 6% lower activity than the wild-type | Loligo vulgaris |
R146S | 45% lower activity than the wild-type | Loligo vulgaris |
S271A | 34% higher activity than the wild-type | Loligo vulgaris |
S271A/D232S | 19% lower activity than the wild-type | Loligo vulgaris |
T195A | 60% lower activity than the wild-type | Loligo vulgaris |
T195L | 11% lower activity than the wild-type | Loligo vulgaris |
T195V | 3% lower activity than the wild-type | Loligo vulgaris |
Y144S | 8% higher activity than the wild-type | Loligo vulgaris |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Loligo vulgaris | Q7SIG4 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Loligo vulgaris |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
4 | - |
mutant N175D | Loligo vulgaris |
7 | - |
mutant N120D | Loligo vulgaris |
19 | - |
mutant H287A | Loligo vulgaris |
31 | - |
mutant F173A | Loligo vulgaris |
63 | - |
mutant F173S | Loligo vulgaris |
78 | - |
mutant T195A | Loligo vulgaris |
84 | - |
mutant H287Y | Loligo vulgaris |
90 | - |
mutant H287Q | Loligo vulgaris |
92 | - |
mutant F173Y | Loligo vulgaris |
97 | - |
mutant Q304F | Loligo vulgaris |
105 | - |
mutant F173V | Loligo vulgaris |
106 | - |
mutant R146S | Loligo vulgaris |
124 | - |
mutant H287F | Loligo vulgaris |
129 | - |
mutant H287W | Loligo vulgaris |
143 | - |
mutant F173L | Loligo vulgaris |
143 | - |
mutant M148A | Loligo vulgaris |
154 | - |
mutant H287L | Loligo vulgaris |
158 | - |
mutant F173W | Loligo vulgaris |
158 | - |
mutant S271A/D232S | Loligo vulgaris |
173 | - |
mutant T195L | Loligo vulgaris |
174 | - |
mutant Q77Y | Loligo vulgaris |
187 | - |
mutant N237S | Loligo vulgaris |
188 | - |
mutant Q304W | Loligo vulgaris |
188 | - |
mutant T195V | Loligo vulgaris |
193 | - |
mutant Q77W | Loligo vulgaris |
194 | - |
wild-type | Loligo vulgaris |
199 | - |
mutant F314A | Loligo vulgaris |
200 | - |
mutant D232S | Loligo vulgaris |
209 | - |
mutant Y144S | Loligo vulgaris |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
diisopropyl fluorophosphate + H2O | room temperature, pH 7.5 | Loligo vulgaris | diisopropyl phosphate + fluoride | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DFPase | - |
Loligo vulgaris |
diisopropylfluorophosphatase | - |
Loligo vulgaris |