Cloned (Comment) | Organism |
---|---|
- |
Loligo vulgaris |
Protein Variants | Comment | Organism |
---|---|---|
H181N | 20% loss of activity in comparison to wild-type enzyme | Loligo vulgaris |
H219N | no effect on catalytic activity | Loligo vulgaris |
H224N | 115% activity in comparison to wild-type enzyme | Loligo vulgaris |
H248N | no effect on catalytic activity | Loligo vulgaris |
H274N | slight loss of activity in comparison to wild-type enzyme using pH Stat measurements, no alteration is observed with fluoride assay | Loligo vulgaris |
H287N | 96% loss of activity in comparison to wild-type enzyme | Loligo vulgaris |
additional information | chemical modification of Tyr, Cys, Arg, Lys, Glu and Asp, this residues are not critical for catalysis | Loligo vulgaris |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | effects of pH, temperature and ionic strength on KM are studied | Loligo vulgaris |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Loligo vulgaris | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
diisopropyl fluorophosphate + H2O = diisopropyl phosphate + fluoride | catalytic mechanism, H287 is required for catalytic activity | Loligo vulgaris |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
pH dependence of the specific activity of the mutant H287N | Loligo vulgaris |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
diisopropyl fluorophosphate + H2O | - |
Loligo vulgaris | diisopropyl phosphate + fluoride | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
208 | - |
diisopropyl fluorophosphate | pH 8, 35°C, effects of pH, temperature and ionic strength on kcat are studied | Loligo vulgaris |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | the effect of pH 6.0-9.0 on kinetic constants kcat and KM is studied, KM shows no perceivable dependence on pH within this pH range, kcat increases with pH to a limiting value at pH 8.0 | Loligo vulgaris |