Cloned (Comment) | Organism |
---|---|
cloning of relSeq fragments and the expression is regulated using pBAD vectors | Streptococcus dysgalactiae subsp. equisimilis |
Protein Variants | Comment | Organism |
---|---|---|
additional information | diverse deletion mutants show synthesis and degradation activities differing from those of full-length enzyme | Streptococcus dysgalactiae subsp. equisimilis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | required | Streptococcus dysgalactiae subsp. equisimilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
48000 | - |
SDS-PAGE | Streptococcus dysgalactiae subsp. equisimilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ppGpp + H2O | Streptococcus dysgalactiae subsp. equisimilis | i.e. guanosine-3',5'-bis(diphosphate) | Gpp + diphosphate | i.e. guanosine 5'-diphosphate | r | |
ppGpp + H2O | Streptococcus dysgalactiae subsp. equisimilis H46A | i.e. guanosine-3',5'-bis(diphosphate) | Gpp + diphosphate | i.e. guanosine 5'-diphosphate | r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptococcus dysgalactiae subsp. equisimilis | - |
H46A | - |
Streptococcus dysgalactiae subsp. equisimilis H46A | - |
H46A | - |
Purification (Comment) | Organism |
---|---|
enzyme fragments | Streptococcus dysgalactiae subsp. equisimilis |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
guanosine 3',5'-bis(diphosphate) + H2O = GDP + diphosphate | RelSeq affects ppGpp synthesis and degradation activities. The C-terminal domain of RelSeq is involved in reciprocal regulation of the two opposing activities present in the N-terminal domain | Streptococcus dysgalactiae subsp. equisimilis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.35 | - |
enzyme fragment NCH | Streptococcus dysgalactiae subsp. equisimilis |
0.46 | - |
enzyme fragment NH 1-24 | Streptococcus dysgalactiae subsp. equisimilis |
0.7 | - |
enzyme fragment NH 1-385 | Streptococcus dysgalactiae subsp. equisimilis |
52 | - |
full-length enzyme NC 1-739 | Streptococcus dysgalactiae subsp. equisimilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ppGpp + H2O | i.e. guanosine-3',5'-bis(diphosphate) | Streptococcus dysgalactiae subsp. equisimilis | Gpp + diphosphate | i.e. guanosine 5'-diphosphate | ? | |
ppGpp + H2O | i.e. guanosine-3',5'-bis(diphosphate) | Streptococcus dysgalactiae subsp. equisimilis | Gpp + diphosphate | i.e. guanosine 5'-diphosphate | r | |
ppGpp + H2O | i.e. guanosine-3',5'-bis(diphosphate) | Streptococcus dysgalactiae subsp. equisimilis H46A | Gpp + diphosphate | i.e. guanosine 5'-diphosphate | ? | |
ppGpp + H2O | i.e. guanosine-3',5'-bis(diphosphate) | Streptococcus dysgalactiae subsp. equisimilis H46A | Gpp + diphosphate | i.e. guanosine 5'-diphosphate | r |
Synonyms | Comment | Organism |
---|---|---|
RelSeq | - |
Streptococcus dysgalactiae subsp. equisimilis |