Literature summary for 3.1.7.11 extracted from

Iijima, Y.; Gang, D.R.; Fridman, E.; Lewinsohn, E.; Pichersky, E.
Characterization of geraniol synthase from the peltate glands of sweet basil (2004), Plant Physiol., 134, 370-379.

Search for more literature for 3.1.7.11

Cloned(Commentary)

Cloned (Comment)	Organism
DNA and amino acid sequence determination and analysis using a GES cDNA, isolated based on analysis of a glandular trichome expressed sequence tag database, sequence comparison, phylogenetic tree, functional expression in Escherichia coli	Ocimum basilicum

Protein Variants

Protein Variants	Comment	Organism
additional information	construction of Ser35 and Met44 truncated GES proteins showing reduced activity compared to the wild-type enzyme	Ocimum basilicum

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.021	-	geranyl diphosphate	pH 8.5, 37°C, wild-type enzyme	Ocimum basilicum
0.029	-	geranyl diphosphate	pH 8.5, 37°C, Ser35 truncated mutant	Ocimum basilicum
0.03	-	geranyl diphosphate	pH 8.5, 37°C, Met44-truncated mutant	Ocimum basilicum

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mn2+	required as a divalent metal cofactor for activity, activating at 0.1-1.0 mM, inhihitory above. Km value of the wild-type enzyme is 0.051 mM at pH 8.5 and 37°C	Ocimum basilicum
additional information	Mg2+ has no effect on the enzyme activity an cannot substitute for Mn2+	Ocimum basilicum

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
57700	-	2 * 57700, native enzyme, SDS-PAGE, 2 * 58600, recombinant enzyme, SDS-PAGE	Ocimum basilicum
58600	-	2 * 57700, native enzyme, SDS-PAGE, 2 * 58600, recombinant enzyme, SDS-PAGE	Ocimum basilicum
140000	-	native enzyme, gel filtration	Ocimum basilicum

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
geranyl diphosphate + H2O	Ocimum basilicum	-	geraniol + diphosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Ocimum basilicum	-	cv. Sweet Dani	-

Purification (Commentary)

Purification (Comment)	Organism
native enzyme 38.3fold from leaves by two differewnt steps of anion exchange chromatography, and by gel filtration	Ocimum basilicum

Reaction

Reaction	Comment	Organism	Reaction ID
geranyl diphosphate + H2O = geraniol + diphosphate	the reaction mechanism of GES is similar to that of other monoterpene synthases and is different from the action of phosphatases	Ocimum basilicum	a

Source Tissue

Source Tissue	Comment	Organism	Textmining
epidermis	-	Ocimum basilicum	-
leaf	younger leaves, which have a higher density of epidermis, also have a higher content of monoterpenes than older leaves	Ocimum basilicum	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
104.1	-	purified native enzyme, pH 8.5, 37°C, wild-type enzyme	Ocimum basilicum

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
geranyl diphosphate + H2O	-	Ocimum basilicum	geraniol + diphosphate	-	?
geranyl diphosphate + H2O	specific reaction	Ocimum basilicum	geraniol + diphosphate	-	?

Subunits

Subunits	Comment	Organism
homodimer	2 * 57700, native enzyme, SDS-PAGE, 2 * 58600, recombinant enzyme, SDS-PAGE	Ocimum basilicum

Synonyms

Synonyms	Comment	Organism
geraniol synthase	-	Ocimum basilicum
GES	-	Ocimum basilicum

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Ocimum basilicum

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
4	20	purified enzyme, 30 min, full activity remaining	Ocimum basilicum
37	-	purified enzyme, 30 min, 80% activity remaining	Ocimum basilicum
45	-	purified enzyme, 30 min, no activity remaining	Ocimum basilicum

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.6	-	geranyl diphosphate	pH 8.5, 37°C, Ser35 truncated mutant	Ocimum basilicum
0.8	-	geranyl diphosphate	pH 8.5, 37°C, wild-type enzyme	Ocimum basilicum
1	-	geranyl diphosphate	pH 8.5, 37°C, Met44-truncated mutant	Ocimum basilicum

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8.5	-	-	Ocimum basilicum

pH Range

pH Minimum	pH Maximum	Comment	Organism
6	7.5	below 10% of maximal activity	Ocimum basilicum
8	9.5	70% of maximal activity at pH 8.0 and pH 9.5	Ocimum basilicum

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Release 2023.1 (January 2023)