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Literature summary for 3.1.6.9 extracted from

  • Sugahara, K.; Kojima, T.
    Specificity studies of bacterial sulfatases by means of structurally defined sulfated oligosaccharides isolated from shark cartilage chondroitin sulfate D (1996), Eur. J. Biochem., 239, 865-870.
    View publication on PubMed

Application

Application Comment Organism
analysis the enzyme is a useful tool for structural studies of chondroitin sulfate and dermetan sulfate Proteus vulgaris

Organism

Organism UniProt Comment Textmining
Proteus vulgaris
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-deoxy-alpha-L-threo-hex-4-enepyranosyluronic acid-beta-(1-3)-GalNAc4SO3--beta-(1-4)-glucuronic acid-2-sulfate-beta-(1-3)-GalNAc6SO3-beta-(1-4)-glucuronic acid-beta-(1-3)-GalNAc4SO3- + H2O removal of two sulfate groups Proteus vulgaris sulfate + 4-deoxy-alpha-L-threo-hex-4-enepyranosyluronic acid-beta-(1-3)-GalNAc-beta-(1-4)-glucuronic acid-2-sulfate-beta-(1-3)-GalNAc6SO3-beta-(1-4)-glucuronic acid-beta-(1-3)-GalNAc
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4-deoxy-alpha-L-threo-hex-4-enepyranosyluronic acid-beta-(1-3)-GalNAc6SO3--beta-(1-4)-glucuronic acid-beta-(1-3)-GalNAc4SO3-beta-(1-4)-glucuronic acid-beta-(1-3)-GalNAc4SO3- + H2O removal of two sulfate groups Proteus vulgaris sulfate + 4-deoxy-alpha-L-threo-hex-4-enepyranosyluronic acid-beta-(1-3)-GalNAc6SO3--beta-(1-4)-glucuronic acid-beta-(1-3)-GalNAc-beta-(1-4)-glucuronic acid-beta-(1-3)-GalNAc
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?
4-deoxy-alpha-L-threo-hex-4-enepyranosyluronic acid-beta-(1-3)-GalNAc6SO3-beta-(1-4)-glucuronic acid-beta-(1-3)-GalNAc6SO3-beta-(1-4)-glucuronic acid-beta-(1-3)-GalNAc4SO3- + H2O removal of one sulfate group Proteus vulgaris sulfate + 4-deoxy-alpha-L-threo-hex-4-enepyranosyluronic acid-beta-(1-3)-GalNAc6SO3-beta-(1-4)-glucuronic acid-beta-(1-3)-GalNAc6SO3-beta-(1-4)-glucuronic acid-beta-(1-3)-GalNAc
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?
4-deoxy-alpha-L-threo-hex-4-enepyranosyluronic acid2SO3--beta-(1-3)-GalNAc6SO3--beta-(1-4)-glucuronic acid-beta-(1-3)-GalNAc4SO3-beta-(1-4)-glucuronic acid-beta-(1-3)-GalNAc4SO3- + H2O removal of two sulfate groups Proteus vulgaris sulfate + 4-deoxy-alpha-L-threo-hex-4-enepyranosyluronic acid2SO3--beta-(1-3)-GalNAc6SO3--beta-(1-4)-glucuronic acid-beta-(1-3)-GalNAc-beta-(1-4)-glucuronic acid-beta-(1-3)-GalNAc
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?
4-deoxy-alpha-L-threo-hex-4-enepyranosyluronic acid2SO3--beta-(1-3)-GalNAc6SO3--beta-(1-4)-glucuronic acid-beta-(1-3)-GalNAc6SO3-beta-(1-4)-glucuronic acid-beta-(1-3)-GalNAc4SO3- + H2O removal of one sulfate group Proteus vulgaris sulfate + 4-deoxy-alpha-L-threo-hex-4-enepyranosyluronic acid2SO3--beta-(1-3)-GalNAc6SO3--beta-(1-4)-glucuronic acid-beta-(1-3)-GalNAc6SO3-beta-(1-4)-glucuronic acid-beta-(1-3)-GalNAc
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additional information the enzyme acts preferentially on an ester sulfate on the C4 position of the GalNAc residue at the reducing end under mild incubation conditions, but also releases a sulfate group under harsh conditions from the C4 position of the GalNAc residue at the internal positions of the hexasaccharide chains, unless the GalNAc residue has another ester sulfate on its C6 position Proteus vulgaris ?
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