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Literature summary for 3.1.6.6 extracted from

  • Jovcic, B.; Venturi, V.; Topisirovic, L.; Kojic, M.
    Inducible expression of choline sulfatase and its regulator BetR in Pseudomonas sp. ATCC19151 (2011), Arch. Microbiol., 193, 399-405.
    View publication on PubMed
Search for more literature for 3.1.6.6

Cloned(Commentary)

Cloned (Comment) Organism
gene betC and DNA of betC and betR intergenic region, DNA and amino acid sequence determination and analysis, the betC promoter is inducible Pseudomonas sp.

Protein Variants

Protein Variants Comment Organism
additional information construction of an enzyme-deficient strain ATCC 19151DELTAbetC and its complementation for choline sulfate utilization by using pLAFRCS cosmid that carries complete choline sulfate operon Pseudomonas sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
choline-O-sulfate + H2O Pseudomonas sp.
-
 choline + sulfate
-
 ?
choline-O-sulfate + H2O Pseudomonas sp. ATCC 19151
-
 choline + sulfate
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas sp. D8LAR6 isolate from sewage with the ability to degrade detergents, e.g. SDS, gene betC
-
Pseudomonas sp. ATCC 19151 D8LAR6 isolate from sewage with the ability to degrade detergents, e.g. SDS, gene betC
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
choline-O-sulfate + H2O
-
 Pseudomonas sp. choline + sulfate
-
 ?
choline-O-sulfate + H2O
-
 Pseudomonas sp. ATCC 19151 choline + sulfate
-
 ?

Synonyms

Synonyms Comment Organism
BetC gene name, the BetC protein belongs to alkPPc superfamily and shares CXPXR sequence with the cysteine sulfatases of group I Pseudomonas sp.
choline sulfatase
-
 Pseudomonas sp.

Expression

Organism Comment Expression
Pseudomonas sp. transcription of betC and betR is not induced in the presence of high concentration of NaCl additional information
Pseudomonas sp. the putative transcriptional regulator BetR activates betC and betR transcription in the presence of choline sulfate, whilst in the absence of choline sulfate BetR represses its own transcription. BetR directly binds to betC-betR intergenic region in vitro, with higher affnity in the presence of choline sulfate as cofactor up

General Information

General Information Comment Organism
metabolism genetic regulation of the enzyme expression, overview Pseudomonas sp.