General Stability | Organism |
---|---|
freezing and thawing five times do not decrease the enzyme activity | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
KCl | - |
Homo sapiens | |
Na2SO4 | - |
Homo sapiens | |
NaCl | - |
Homo sapiens | |
NaH2PO4 | - |
Homo sapiens | |
phosphate | - |
Homo sapiens | |
SO42- | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.008 | - |
galactosamine 6-sulfate | - |
Homo sapiens | |
0.013 | - |
Galactose 6-sulfate | - |
Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
15000 | - |
x * 15000 + x * 40000, the 15000 Da subunit is a glycoprotein, SDS-PAGE | Homo sapiens |
40000 | - |
x * 15000 + x * 40000, the 15000 Da subunit is a glycoprotein, SDS-PAGE | Homo sapiens |
120000 | - |
gel filtration | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | the 15000 Da subunit is a glycoprotein | Homo sapiens |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
placenta | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.13 | - |
- |
Homo sapiens |
Storage Stability | Organism |
---|---|
-20°C, 2 months, 5% loss of activity | Homo sapiens |
4°C, 2 months, 20% loss of activity | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-N-acetyl-D-galactosamine-6-sulfate-(1-4)-beta-D-glucuronic acid-(1-3)-N-acetyl-D-galactosaminitol 6-sulfate + H2O | - |
Homo sapiens | sulfate + ? | - |
? | |
galactosamine 6-sulfate + H2O | - |
Homo sapiens | galactosamine + sulfate | - |
? | |
galactose 6-sulfate + H2O | - |
Homo sapiens | galactose + sulfate | - |
? | |
N-acetylgalactosamine 6-sulfate + H2O | - |
Homo sapiens | N-acetylgalactosamine + sulfate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 15000 + x * 40000, the 15000 Da subunit is a glycoprotein, SDS-PAGE | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
3.8 | 4 | hydrolysis of N-acetylgalactosamine 6-sulfate or galactose 6-sulfate | Homo sapiens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3.3 | 4.2 | pH 3.0: about 45% of maximal activity, pH 4.2: about 55% of maximal activity, hydrolysis of N-acetylgalactosamine 6-sulfate, 0.02 M acetate buffer | Homo sapiens |