Cloned (Comment) | Organism |
---|---|
His6-tagged wild-type STS cDNA subcloned into pCEP4, transfected into 293-EBNA cells, cell line with stable overexpression of STS-His6 selected for production of tagged wildtype STS, subsequent specific antibody production, mutated STS is transfected into human MCF-7 cells and monkey COS-1 cells to test for STS activity by immunoblotting | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
DELTA1-21 | lacks 21 N-terminal amino acids, that encode the signal peptide, reduced STS activity | Homo sapiens |
DELTA185-583 | lacks 399 C-terminal amino acids, reduced STS activity | Homo sapiens |
H136F | point mutation in histidine 136, reduced STS activitiy | Homo sapiens |
H136W | point mutation in histidine 136, reduced STS activity | Homo sapiens |
H136Y | point mutation in histidine 136, reduced STS activity | Homo sapiens |
additional information | site directed mutagenisis is introduced using pALTERMax kit | Homo sapiens |
N259Q | point mutation in asparagine 259 glycosylation site, reduced STS activity | Homo sapiens |
N333Q | point mutation in other potential glycosylation site asparagine 333, no reduced STS activity | Homo sapiens |
N459Q | point mutation in other potential glycosylation site asparagine 459, no reduced STS activity | Homo sapiens |
N47Q | point mutation in asparagine 47 glycosylation site, reduced STS activity | Homo sapiens |
N47Q/N259Q | double mutation in asparagine 47 and 259 glycosylation sites, reduced STS activity | Homo sapiens |
N54Q | point mutation in in asparagine 54 non-glycosylation site , no reduced STS activity | Homo sapiens |
P212G | point mutation in proline 212, no reduced STS activity | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endoplasmic reticulum | - |
Homo sapiens | 5783 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
61000 | - |
SDS-PAGE, immunoblot analysis with specific antibody | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
estrone sulfate | Homo sapiens | - |
estrone + sulfate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P08842 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | 4 potential glycosylation sites | Homo sapiens |
Purification (Comment) | Organism |
---|---|
affinity chromatography with NiCl2-primed HisTrap Chelating columns and concentrated 6-fold using ICON concentrators | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
HEK-293-EBNA cell | human embryonic kidney carcinoma cell line selected for overexpression of transfected His6-tagged STS cDNA to aid purification of the His6-tagged STS protein and subsequent antibody production in mice and rabbits | Homo sapiens | - |
JEG-3 cell | choriocarcinoma cell line used for expression test for transfection experiments | Homo sapiens | - |
MCF-7 cell | ER positive human breast cancer cells, used for transfection and activity experiments, shows STS activity by itself in contrast to monkey COS-1 cells used for the same experiments | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
estrone sulfate | - |
Homo sapiens | estrone + sulfate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
steroid sulfatase | - |
Homo sapiens |
STS | - |
Homo sapiens |