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Literature summary for 3.1.6.2 extracted from

  • Stengel, C.; Newman, S.P.; Day, J.M.; Tutill, H.J.; Reed, M.J.; Purohit, A.
    Effects of mutations and glycosylations on STS activity: a site-directed mutagenesis study (2008), Mol. Cell. Endocrinol., 283, 76-82.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
His6-tagged wild-type STS cDNA subcloned into pCEP4, transfected into 293-EBNA cells, cell line with stable overexpression of STS-His6 selected for production of tagged wildtype STS, subsequent specific antibody production, mutated STS is transfected into human MCF-7 cells and monkey COS-1 cells to test for STS activity by immunoblotting Homo sapiens

Protein Variants

Protein Variants Comment Organism
DELTA1-21 lacks 21 N-terminal amino acids, that encode the signal peptide, reduced STS activity Homo sapiens
DELTA185-583 lacks 399 C-terminal amino acids, reduced STS activity Homo sapiens
H136F point mutation in histidine 136, reduced STS activitiy Homo sapiens
H136W point mutation in histidine 136, reduced STS activity Homo sapiens
H136Y point mutation in histidine 136, reduced STS activity Homo sapiens
additional information site directed mutagenisis is introduced using pALTERMax kit Homo sapiens
N259Q point mutation in asparagine 259 glycosylation site, reduced STS activity Homo sapiens
N333Q point mutation in other potential glycosylation site asparagine 333, no reduced STS activity Homo sapiens
N459Q point mutation in other potential glycosylation site asparagine 459, no reduced STS activity Homo sapiens
N47Q point mutation in asparagine 47 glycosylation site, reduced STS activity Homo sapiens
N47Q/N259Q double mutation in asparagine 47 and 259 glycosylation sites, reduced STS activity Homo sapiens
N54Q point mutation in in asparagine 54 non-glycosylation site , no reduced STS activity Homo sapiens
P212G point mutation in proline 212, no reduced STS activity Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum
-
Homo sapiens 5783
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
61000
-
SDS-PAGE, immunoblot analysis with specific antibody Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
estrone sulfate Homo sapiens
-
estrone + sulfate
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens P08842
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein 4 potential glycosylation sites Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
affinity chromatography with NiCl2-primed HisTrap Chelating columns and concentrated 6-fold using ICON concentrators Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
HEK-293-EBNA cell human embryonic kidney carcinoma cell line selected for overexpression of transfected His6-tagged STS cDNA to aid purification of the His6-tagged STS protein and subsequent antibody production in mice and rabbits Homo sapiens
-
JEG-3 cell choriocarcinoma cell line used for expression test for transfection experiments Homo sapiens
-
MCF-7 cell ER positive human breast cancer cells, used for transfection and activity experiments, shows STS activity by itself in contrast to monkey COS-1 cells used for the same experiments Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
estrone sulfate
-
Homo sapiens estrone + sulfate
-
?

Synonyms

Synonyms Comment Organism
steroid sulfatase
-
Homo sapiens
STS
-
Homo sapiens