Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-Chloropropionate | 20 mM, 8% inhibition | Pseudomonas syringae | |
butan-2-yl-sulfate | 20 mM, 25% inhibition | Pseudomonas syringae | |
butyrate-2-sulfate | 20 mM, 42% inhibition | Pseudomonas syringae | |
D-lactate | product inhibition, kinetics | Pseudomonas syringae | |
D-serine-O-sulfate | 20 mM, 11% inhibition | Pseudomonas syringae | |
glycolate-2-sulfate | 20 mM, 43% inhibition | Pseudomonas syringae | |
Hexan-2-yl sulfate | 20 mM, 13% inhibition | Pseudomonas syringae | |
L-lactate 2-sulfate | 12 mM, 43% inhibition | Pseudomonas syringae | |
pentan-3-yl hydrogen sulfate | 20 mM, 10% inhibition | Pseudomonas syringae | |
propan-1-yl sulfate | 20 mM, 22% inhibition | Pseudomonas syringae | |
propan-2-yl-sulfate | 20 mM, 18% inhibition | Pseudomonas syringae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0067 | - |
D-Lactate 2-sulfate | pH 7.5, 25°C | Pseudomonas syringae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Pseudomonas syringae | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
1 * 55000, Pseudomonas syringae, SDS-PAGE | Pseudomonas syringae |
59800 | - |
PAGE, gel filtration yields a higher MW, presumably due to aggregation | Pseudomonas syringae |
89000 | - |
gel filtration, high MW presumably due to aggregation | Pseudomonas syringae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas syringae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas syringae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
10.9 | - |
pH 7.5, 25°C | Pseudomonas syringae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl sulfate + H2O | at 6% the rate of D-lactate 2-sulfate hydrolysis | Pseudomonas syringae | 4-nitrophenol + SO42- | - |
? | |
D-lactate 2-sulfate + H2O | stereospecific, not L-lactate 2-sulfate, cleaves O-S-bond of the C-O-S-ester link | Pseudomonas syringae | D-lactate + SO42- | not L-lactate | ? | |
additional information | no substrates are the sulfate esters of L-lactate, methanol, ethanol, propanol, propan-2-ol, butanol, butan-2-ol, pentanol, pentan-2-ol, pentan-3-ol, hexanol, hexan-2-ol, heptanol, heptan-2-ol, heptan-4-ol, octanol, octan-2-ol, decanol, decan-5-ol, 2-hydroxybutyrate, 2-hydroxyisobutyrate, butoxyethanol and phenoxyethanol, the 6-sulfate esters of glucosamine and N-acetylglucosamine and the 3-sulfate esters of glucosamine and N-acetylgalactosamine. No dehalogenase activity: potassium DL-chloropropionate is not a substrate | Pseudomonas syringae | ? | - |
? | |
serine-O-sulfate + H2O | at 9% the rate of D-lactate 2-sulfate hydrolysis | Pseudomonas syringae | serine + SO42- | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 55000, Pseudomonas syringae, SDS-PAGE | Pseudomonas syringae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
- |
Pseudomonas syringae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
pH 7.5, stable up to, 5 min | Pseudomonas syringae |
50 | - |
pH 7.5, 5 min, inactivation | Pseudomonas syringae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
0.2 M Tris-HCl buffer | Pseudomonas syringae |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
16.3 | - |
L-lactate-2-sulfate | pH 7.5, 25°C | Pseudomonas syringae |