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Literature summary for 3.1.6.17 extracted from

  • Crescenzi, A.M.V.; Dodgson, K.S.; White, G.F.
    Purification and some properties of the D-lactate-2-sulphatase of Pseudomonas syringae GG (1984), Biochem. J., 223, 487-494.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
2-Chloropropionate 20 mM, 8% inhibition Pseudomonas syringae
butan-2-yl-sulfate 20 mM, 25% inhibition Pseudomonas syringae
butyrate-2-sulfate 20 mM, 42% inhibition Pseudomonas syringae
D-lactate product inhibition, kinetics Pseudomonas syringae
D-serine-O-sulfate 20 mM, 11% inhibition Pseudomonas syringae
glycolate-2-sulfate 20 mM, 43% inhibition Pseudomonas syringae
Hexan-2-yl sulfate 20 mM, 13% inhibition Pseudomonas syringae
L-lactate 2-sulfate 12 mM, 43% inhibition Pseudomonas syringae
pentan-3-yl hydrogen sulfate 20 mM, 10% inhibition Pseudomonas syringae
propan-1-yl sulfate 20 mM, 22% inhibition Pseudomonas syringae
propan-2-yl-sulfate 20 mM, 18% inhibition Pseudomonas syringae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0067
-
D-Lactate 2-sulfate pH 7.5, 25°C Pseudomonas syringae

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
Pseudomonas syringae
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
1 * 55000, Pseudomonas syringae, SDS-PAGE Pseudomonas syringae
59800
-
PAGE, gel filtration yields a higher MW, presumably due to aggregation Pseudomonas syringae
89000
-
gel filtration, high MW presumably due to aggregation Pseudomonas syringae

Organism

Organism UniProt Comment Textmining
Pseudomonas syringae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Pseudomonas syringae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
10.9
-
pH 7.5, 25°C Pseudomonas syringae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl sulfate + H2O at 6% the rate of D-lactate 2-sulfate hydrolysis Pseudomonas syringae 4-nitrophenol + SO42-
-
?
D-lactate 2-sulfate + H2O stereospecific, not L-lactate 2-sulfate, cleaves O-S-bond of the C-O-S-ester link Pseudomonas syringae D-lactate + SO42- not L-lactate ?
additional information no substrates are the sulfate esters of L-lactate, methanol, ethanol, propanol, propan-2-ol, butanol, butan-2-ol, pentanol, pentan-2-ol, pentan-3-ol, hexanol, hexan-2-ol, heptanol, heptan-2-ol, heptan-4-ol, octanol, octan-2-ol, decanol, decan-5-ol, 2-hydroxybutyrate, 2-hydroxyisobutyrate, butoxyethanol and phenoxyethanol, the 6-sulfate esters of glucosamine and N-acetylglucosamine and the 3-sulfate esters of glucosamine and N-acetylgalactosamine. No dehalogenase activity: potassium DL-chloropropionate is not a substrate Pseudomonas syringae ?
-
?
serine-O-sulfate + H2O at 9% the rate of D-lactate 2-sulfate hydrolysis Pseudomonas syringae serine + SO42-
-
?

Subunits

Subunits Comment Organism
monomer 1 * 55000, Pseudomonas syringae, SDS-PAGE Pseudomonas syringae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
Pseudomonas syringae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25
-
pH 7.5, stable up to, 5 min Pseudomonas syringae
50
-
pH 7.5, 5 min, inactivation Pseudomonas syringae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
0.2 M Tris-HCl buffer Pseudomonas syringae

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
16.3
-
L-lactate-2-sulfate pH 7.5, 25°C Pseudomonas syringae