Literature summary for 3.1.6.13 extracted from

Sosa, A.C.; Espejo, A.J.; Rodriguez, E.A.; Lizaraso, L.M.; Rojas, A.; Guevara, J.; Echeverri, O.Y.; Barrera, L.A.
Development of a sandwich enzyme linked immunosorbent assay (ELISA) for the quantification of iduronate-2-sulfate sulfatase (2011), J. Immunol. Methods, 368, 64-70.

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Cloned(Commentary)

Cloned (Comment)	Organism
development of an expression system for human recombinant IDS in Pichia pastoris and of a detection method for enzyme detection during production and purification processes, which can be used also to measure the enzyme in human fluids, immunoquantification assay using rabbit IgG and chicken IgY, overview	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
commercial preparation	recombinant enzyme	Homo sapiens	-
additional information	development of an ELISA system to detect and quantify IDS with IgY antibodies, overview	Homo sapiens	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.000006	0.00066	hrIDS detection by ELISA-sandwich and fluorometric assay in Pichia pastoris culture samples, substrate is 4-methylumbelliferyl-2-iduronate-2-sulfate, pH not specified in the publication, temperature not specified in the publication, overview	Homo sapiens
0.00063	0.0021	enzyme determination in human plasma samples by a Fluorometric method, and ELISA double sandwich technique after several freeze and thawing cycles, substrate is 4-methylumbelliferyl-2-iduronate-2-sulfate, pH not specified in the publication, temperature not specified in the publication, overview	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-methylumbelliferyl-alpha-L-iduronate 2-sulfate + H2O	artificial substrate	Homo sapiens	4-methylumbelliferol + L-iduronate 2-sulfate	-	?

Synonyms

Synonyms	Comment	Organism
iduronate-2-sulfate sulfatase	-	Homo sapiens

General Information

General Information	Comment	Organism
malfunction	iduronate-2-sulfate sulfatase, IDS, deficiency causes the Hunter syndrome ormucopolysaccharidosis type II	Homo sapiens
additional information	development of an expression system for human recombinant IDS in Pichia pastoris and of a detection method for enzyme detection during production and purification processes, which can be used also to measure the enzyme in human fluids, immunoquantification assay using rabbit IgG and chicken IgY, overview	Homo sapiens

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Release 2023.1 (January 2023)