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Literature summary for 3.1.6.13 extracted from

  • Lu, J.Z.; Hui, E.K.; Boado, R.J.; Pardridge, W.M.
    Genetic engineering of a bifunctional IgG fusion protein with iduronate-2-sulfatase (2010), Bioconjug. Chem., 21, 151-156.
    View publication on PubMed
Search for more literature for 3.1.6.13

Application

Application Comment Organism
drug development HIRMAb-IDS fusion protein is a bifunctional IgG-sulfatase fusion protein, specifically engineered for targeted drug delivery across the human blood-brain barrier Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
HIRMAb-IDS fusion protein (IDS cDNA, encoding Ser26-Pro550, minus the 25 amino acid signal peptide, fused to the carboxyl terminus of the heavy chain of a chimeric monoclonal antibody to the human insulin receptor) expressed in COS cells Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
 the fusion protein is about 80 kDa larger than the heavy chain of the chimeric HIRMAb Homo sapiens
108000
-
 heavy-chain HIRMAb-IDS fusion protein, calculated from sequence Homo sapiens
135000
-
 heavy-chain HIRMAb-IDS fusion protein, Western blotting Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
HIRMAb-IDS fusion protein purified by protein A affinity chromatography, to homogeneity Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl-alpha-L-iduronide-2-sulfate + H2O
-
 Homo sapiens 4-methylumbelliferyl-alpha-L-iduronide + sulfate
-
 ?

Subunits

Subunits Comment Organism
heterotetramer HIRMAb-IDS fusion protein Homo sapiens

Synonyms

Synonyms Comment Organism
IDS
-
 Homo sapiens
iduronate-2-sulfatase
-
 Homo sapiens

pI Value

Organism Comment pI Value Maximum pI Value
Homo sapiens heavy-chain HIRMAb-IDS fusion protein, calculated from sequence
-
 6.03

General Information

General Information Comment Organism
physiological function HIRMAb-IDS fusion protein crosses the blood-brain barrier on the endogenous insulin receptor and acts as a molecular Trojan horse to ferry the IDS into brain. The fusion protein is taken up by Hunter fibroblasts, and the accumulation of glycosoaminoglycans in fibroblasts null for the sulfatase is decreased 84% by treatment with the fusion protein. The fusion protein heavy chain reacts with antibodies to both human IgG and human IDS. The fusion protein is a bifunctional molecule and binds both the HIR extracellular domain with comparable affinity to the chimeric HIRMAb and has high IDS enzyme activity Homo sapiens