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Literature summary for 3.1.6.12 extracted from

  • Yaghootfam, A.; Schestag, F.; Dierks, T.; Gieselmann, V.
    Recognition of arylsulfatase A and B by the UDP-N-acetylglucosamine:lysosomal enzyme N-acetylglucosamine-phosphotransferase (2003), J. Biol. Chem., 278, 32653-32661.
    View publication on PubMed
Search for more literature for 3.1.6.12

Cloned(Commentary)

Cloned (Comment) Organism
wild-type and mutant enzymes transiently transfected into BHK cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
K470A mutation leads to an decrease of enzyme activity in the medium to 13% of total activity compared to 25% of the wild-type enzyme, no effect on phosphorylation Homo sapiens
K470A/K497A/K507A mutation leads to an decrease of enzyme activity in the medium to 17% of total activity compared to 25% of the wild-type enzyme, no effect on phosphorylation Homo sapiens
K470A/K507A mutation leads to an decrease of enzyme activity in the medium to 16% of total activity compared to 25% of the wild-type enzyme, no effect on phosphorylation Homo sapiens
K497A mutation leads to an decrease of enzyme activity in the medium to 19% of total activity compared to 25% of the wild-type enzyme, no effect on phosphorylation Homo sapiens
K497A/K507A mutation leads to an decrease of enzyme activity in the medium to 18% of total activity compared to 25% of the wild-type enzyme, no effect on phosphorylation Homo sapiens
K507A mutation leads to an decrease of enzyme activity in the medium to 23% of total activity compared to 25% of the wild-type enzyme, no effect on phosphorylation Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
lysosome
-
 Homo sapiens 5764
-

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein the wild-type enzyme has three N-glycosylation sites. Only oligosaccharides at the first, Asn158, and the third, Asn350, glycosylation site are phosphorylated, whereas the second, Asn184 is not Homo sapiens
phosphoprotein compared to degree of mannose-phosphorylation of the wild-type enzyme the mutant enzyme K457R has 33%, mutant enzyme K457S has 50%, mutant enzyme K457G has 31% mutant enzyme K433A has 95%, mutant enzyme K367A has 106% and mutant enzyme K393A has 123% of mannose-phosphorylation Homo sapiens

Synonyms

Synonyms Comment Organism
ASB
-
 Homo sapiens