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Literature summary for 3.1.6.12 extracted from

  • Habuchi, H.; Tsuji, M.; Nakanishi, Y.; Suzuki, S.
    Separation and properties of five glycosaminoglycan sulfatases from rat skin (1979), J. Biol. Chem., 254, 7570-7578.
    View publication on PubMed
Search for more literature for 3.1.6.12

Inhibitors

Inhibitors Comment Organism Structure
KCl at 10 mM Rattus norvegicus
Mg2+
-
 Rattus norvegicus
Mn2+
-
 Rattus norvegicus
NaCl at 10 mM Rattus norvegicus
SO42-
-
 Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.027
-
 N-acetylgalactosamine 4-sulfate-beta-glucuronosyl-(1-3)-N-acetylgalactosamine 4-sulfate
-
 Rattus norvegicus
0.03
-
 N-acetylgalactosamine 4-sulfate-(beta-glucuronosyl-(1-3)-N-acetylgalactosamine 4-sulfate)2
-
 Rattus norvegicus
0.049
-
 N-acetylgalactosamine 4-sulfate-(beta-glucuronosyl-(1-3)-N-acetylgalactosamine 4-sulfate)3
-
 Rattus norvegicus
0.064
-
 N-acetylgalactosamine 4-sulfate-(beta-glucuronosyl-(1-3)-N-acetylgalactosamine 4-sulfate)4
-
 Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
lysosome
-
 Rattus norvegicus 5764
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N-acetyl-D-galactosamine 4-sulfate units + H2O Rattus norvegicus acts also on N-acetylglucosamine 4-sulfate N-acetyl-D-galactosamine units + sulfate
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
skin
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chondroitin 4-sulfate + H2O
-
 Rattus norvegicus chondroitin + sulfate
-
 ?
chondroitin 4-sulfate-heptasaccharide + H2O
-
 Rattus norvegicus chondroitin 4-sulfate-heptasaccharide + sulfate
-
 ?
dermatan sulfate + H2O
-
 Rattus norvegicus dermatan + sulfate
-
 ?
N-acetyl-D-galactosamine 4-sulfate + H2O
-
 Rattus norvegicus N-acetyl-D-galactosamine + sulfate
-
 ?
N-acetyl-D-galactosamine 4-sulfate units + H2O acts also on N-acetylglucosamine 4-sulfate Rattus norvegicus N-acetyl-D-galactosamine units + sulfate
-
 ?
N-acetylgalactosamine 4-sulfate-(beta-glucuronosyl-(1-3)-N-acetylgalactosamine 4-sulfate)2 + H2O only the 4-sulfated endgroup is attacked by the enzyme Rattus norvegicus N-acetylgalactosamine-(beta-glucuronosyl-(1-3)-N-acetylgalactosamine 4-sulfate)2 + sulfate
-
 ?
N-acetylgalactosamine 4-sulfate-(beta-glucuronosyl-(1-3)-N-acetylgalactosamine 4-sulfate)3 + H2O only the 4-sulfated endgroup is attacked by the enzyme Rattus norvegicus N-acetylgalactosamine-(beta-glucuronosyl-(1-3)-N-acetylgalactosamine)3 + sulfate
-
 ?
N-acetylgalactosamine 4-sulfate-(beta-glucuronosyl-(1-3)-N-acetylgalactosamine 4-sulfate)4 + H2O only the 4-sulfated endgroup is attacked by the enzyme Rattus norvegicus N-acetylgalactosamine-(beta-glucuronosyl-(1-3)-N-acetylgalactosamine 4-sulfate)4 + sulfate
-
 ?
N-acetylgalactosamine 4-sulfate-beta-glucuronosyl-(1-3)-N-acetylgalactosamine 4-sulfate + H2O
-
 Rattus norvegicus N-acetylgalactosamine-beta-glucuronosyl-(1-3)-N-acetylgalactosamine 4-sulfate + sulfate
-
 ?
N-acetylglucosamine 4-sulfate + H2O
-
 Rattus norvegicus N-acetylglucosamine + sulfate
-
 ?
p-nitrocatechol sulfate + H2O
-
 Rattus norvegicus p-nitrocatechol + sulfate
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4 4.8 substrate-dependent Rattus norvegicus