Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | - |
Homo sapiens | |
EDTA | 60% activation at 10 mM | Homo sapiens |
General Stability | Organism |
---|---|
freezing, purified enzyme, rapid loss of activity, after 24 h irreversible | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Homo sapiens | |
Cu2+ | bivalent cations are inhibitory | Homo sapiens | |
iodoacetate | - |
Homo sapiens | |
Mg2+ | - |
Homo sapiens | |
Mn2+ | slight | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.8 | - |
nitrocatechol sulfate | - |
Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
47000 | 54000 | gel filtration | Homo sapiens |
57000 | 61000 | sedimentation equilibrium | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-acetyl-D-galactosamine 4-sulfate units + H2O | Homo sapiens | acts also on N-acetylglucosamine 4-sulfate | N-acetyl-D-galactosamine units + sulfate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Oxidation Stability | Organism |
---|---|
photooxidation, with 1 mM Rose Bengal, 80% inactivation, no inactivation in presence of 1 mM pyridoxal phosphate and 30 mM 4-nitrocatechol | Homo sapiens |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-acetyl-D-galactosamine 4-sulfate units + H2O | acts also on N-acetylglucosamine 4-sulfate | Homo sapiens | N-acetyl-D-galactosamine units + sulfate | - |
? | |
p-nitrocatechol sulfate + H2O | enzymes catalyze in addition to reaction of EC 3.1.6.12 also arylsulfatase reaction , see also EC 3.1.6.1 | Homo sapiens | p-nitrocatechol + sulfate | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.1 | - |
nitrocatechol sulfate | Homo sapiens |