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Literature summary for 3.1.6.1 extracted from
- Constitutive overexpression of Pseudoalteromonas carrageenovora arylsulfatase in E. coli fed-batch culture (2011), Korean J. Chem. Engin., 28, 1101-1104.
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| astA gene from Pseudoalteromonas carrageenovora genome is subcloned into pHCEIA vector employing the hyper constitutive expression promoter from the D-amino acid aminotransferase gene of Geobacillus toebii. When the cell is cultured on fermentor containing MaxyBroth-HD medium with 1% glycerol, the enzyme activity reaches 12.8 micromol/min/mL. On MaxyBroth-HD medium with 2% glycerol, the cell shows 2.7fold higher arylsulfatase expression than that with 1% glycerol. The fed-batch cultivation employing MaxyBroth-HD medium and additional feeding of glycerol gave about 143 micromol/min/mL of arylsulfatase at 20 h, which corresponds to 4fold higher enzyme activity than that of 2% glycerol batch culture | Pseudoalteromonas carrageenovora |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudoalteromonas carrageenovora | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl sulfate + H2O | - |
Pseudoalteromonas carrageenovora | 4-nitrophenol + sulfate | - |
? |  |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| arylsulfatase gene | - |
Pseudoalteromonas carrageenovora |
| AstA | - |
Pseudoalteromonas carrageenovora |
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Release 2023.1 (January 2023)
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