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Literature summary for 3.1.6.1 extracted from

  • Stawoska, I.; Gawda, S.; Bielak-Lakomska, M.; Brindell, M.; Lewinski, K.; Laidler, P.; Stochel, G.
    Mechanistic studies of the hydrolysis of p-nitrophenyl sulfate catalyzed by arylsulfatase from Helix pomatia (2010), J. Coord. Chem., 63, 2472-2487.
No PubMed abstract available
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KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.82
-
 4-nitrophenyl sulfate pH 7.4, 29.85°C, pressure: 160 MPa Helix pomatia
1.12
-
 4-nitrophenyl sulfate pH 7.4, 29.85°C, pressure: 120 MPa Helix pomatia
1.2
-
 4-nitrophenyl sulfate pH 7.08, 30°C Helix pomatia
1.5 2 4-nitrophenyl sulfate pH 7.4, 24.85°C, pressure: 0.1 MPa Helix pomatia
1.6
-
 4-nitrophenyl sulfate pH 7.4, 29.85°C, pressure: 5 MPa Helix pomatia
1.61
-
 4-nitrophenyl sulfate pH 7.4, 29.85°C, pressure: 40 MPa Helix pomatia
1.62
-
 4-nitrophenyl sulfate pH 7.4, 29.85°C, pressure: 80 MPa Helix pomatia
1.85
-
 4-nitrophenyl sulfate pH 7.43, 30°C Helix pomatia
2.01
-
 4-nitrophenyl sulfate pH 7.4, 19.85°C, pressure: 0.1 MPa Helix pomatia
2.12
-
 4-nitrophenyl sulfate pH 7.4, 29.85°C, pressure: 0.1 MPa Helix pomatia
2.31
-
 4-nitrophenyl sulfate pH 7.4, 22.35°C, pressure: 0.1 MPa Helix pomatia
2.47
-
 4-nitrophenyl sulfate pH 7.4, 14.85°C, pressure: 0.1 MPa Helix pomatia
2.49
-
 4-nitrophenyl sulfate pH 7.4, 17.35°C, pressure: 0.1 MPa Helix pomatia
2.5
-
 4-nitrophenyl sulfate pH 7.4, 34.85°C, pressure: 0.1 MPa Helix pomatia
4.36
-
 4-nitrophenyl sulfate pH 8.08, 30°C Helix pomatia
5.26
-
 4-nitrophenyl sulfate pH 8.68, 30°C Helix pomatia

Organism

Organism UniProt Comment Textmining
Helix pomatia
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl sulfate + H2O
-
 Helix pomatia 4-nitrophenol + sulfate
-
 ?

Synonyms

Synonyms Comment Organism
arylsulfatase
-
 Helix pomatia

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Helix pomatia

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
40.5
-
 4-nitrophenyl sulfate pH 7.4, 29.85°C, pressure: 120 MPa Helix pomatia
41.83
-
 4-nitrophenyl sulfate pH 7.4, 17.35°C, pressure: 0.1 MPa Helix pomatia
42.5
-
 4-nitrophenyl sulfate pH 7.4, 29.85°C, pressure: 160 MPa Helix pomatia
42.83
-
 4-nitrophenyl sulfate pH 7.4, 14.85°C, pressure: 0.1 MPa Helix pomatia
46.16
-
 4-nitrophenyl sulfate pH 7.4, 19.85°C, pressure: 0.1 MPa Helix pomatia
46.5
-
 4-nitrophenyl sulfate pH 7.4, 29.85°C, pressure: 40 MPa Helix pomatia
48.33
-
 4-nitrophenyl sulfate pH 7.4, 29.85°C, pressure: 5 MPa Helix pomatia
51.33
-
 4-nitrophenyl sulfate pH 7.4, 29.85°C, pressure: 80 MPa Helix pomatia
56.33
-
 4-nitrophenyl sulfate pH 7.4, 24.85°C, pressure: 0.1 MPa Helix pomatia
58
-
 4-nitrophenyl sulfate pH 7.4, 22.35°C, pressure: 0.1 MPa Helix pomatia
74.5
-
 4-nitrophenyl sulfate pH 7.4, 29.85°C, pressure: 0.1 MPa Helix pomatia
90.83
-
 4-nitrophenyl sulfate pH 7.4, 34.85°C, pressure: 0.1 MPa Helix pomatia

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Helix pomatia