Protein Variants | Comment | Organism |
---|---|---|
C51A | mutant with strongly reduced activity | Pseudomonas aeruginosa |
C51S | mutant with strongly reduced activity | Pseudomonas aeruginosa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-nitrophenyl phosphate | - |
Pseudomonas aeruginosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00025 | - |
4-nitrophenyl sulfate | mutant enzyme C51S, in 100 mM Tris, pH 8.0, 25°C | Pseudomonas aeruginosa | |
0.00029 | - |
4-nitrophenyl sulfate | wild type enzyme, in 100 mM Tris, pH 8.0, 25°C | Pseudomonas aeruginosa | |
0.0004 | - |
4-nitrophenyl sulfate | mutant enzyme C51A, in 100 mM Tris, pH 8.0, 25°C | Pseudomonas aeruginosa | |
0.0291 | - |
4-nitrophenyl phosphate | wild type enzyme, in 100 mM Tris, pH 8.0, 25°C | Pseudomonas aeruginosa | |
0.0752 | - |
4-nitrophenyl phosphate | mutant enzyme C51S, in 100 mM Tris, pH 8.0, 25°C | Pseudomonas aeruginosa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
SDS-PAGE | Pseudomonas aeruginosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl phosphate + H2O | - |
Pseudomonas aeruginosa | 4-nitrophenol + phosphate | - |
? | |
4-nitrophenyl sulfate + H2O | - |
Pseudomonas aeruginosa | 4-nitrophenol + sulfate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PAS | - |
Pseudomonas aeruginosa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0000095 | - |
4-nitrophenyl phosphate | mutant enzyme C51S, in 100 mM Tris, pH 8.0, 25°C | Pseudomonas aeruginosa | |
0.000061 | - |
4-nitrophenyl sulfate | mutant enzyme C51A, in 100 mM Tris, pH 8.0, 25°C | Pseudomonas aeruginosa | |
0.0054 | - |
4-nitrophenyl sulfate | mutant enzyme C51S, in 100 mM Tris, pH 8.0, 25°C | Pseudomonas aeruginosa | |
0.023 | - |
4-nitrophenyl phosphate | wild type enzyme, in 100 mM Tris, pH 8.0, 25°C | Pseudomonas aeruginosa | |
14.2 | - |
4-nitrophenyl sulfate | wild type enzyme, in 100 mM Tris, pH 8.0, 25°C | Pseudomonas aeruginosa |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0292 | - |
4-nitrophenyl phosphate | wild type enzyme, in 100 mM Tris, pH 8.0, 25°C | Pseudomonas aeruginosa |