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Literature summary for 3.1.6.1 extracted from

  • Droba, M.; Droba, B.
    Further studies on arylsulphatase activity from fowl seminal plasma (1983), Folia Biol. (Krakow), 31, 107-117.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
AgNO3 0.2 mM, 20-30% inhibition Gallus gallus
K2HPO4 5 mM, 97-98% inhibition Gallus gallus
NaCl 125 mM, 20-30% inhibition Gallus gallus
SO42- 12.5 mM K2SO4, 80-85% inhibition Gallus gallus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.2
-
4-nitrocatechol sulfate enzyme from fraction II and fraction III Gallus gallus
0.23
-
4-nitrocatechol sulfate enzyme from fraction I Gallus gallus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
59000
-
enzyme from fraction II, gel filtration Gallus gallus
94000
-
enzyme from fraction I, gel filtration Gallus gallus
105000
-
enzyme from fraction III, gel filtration Gallus gallus

Organism

Organism UniProt Comment Textmining
Gallus gallus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
seminal plasma
-
Gallus gallus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-hydroxy-5-nitrophenyl sulfate + H2O
-
Gallus gallus 2-hydroxy-5-nitrophenol + sulfate
-
?
4-nitrocatechol sulfate + H2O
-
Gallus gallus 4-nitrocatechol + sulfate
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5 5.6 pH optimum gradually changes from pH 5.6 to 5.0 when the time of incubation is prolonged from 5 to 30-120 min Gallus gallus
5.4
-
120 min incubation in presence of NaCl Gallus gallus
5.8
-
10 min incubation in presence of NaCl Gallus gallus
6
-
120 min incubation in presence of K2SO4 Gallus gallus
6.2
-
10 min incubation in presence of K2SO4 Gallus gallus