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Literature summary for 3.1.6.1 extracted from
- Arylsulfatase from Streptomyces griseorubiginosus S980-14 (1995), Biosci. Biotechnol. Biochem., 59, 1062-1068.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| (NH4)2SO4 | - |
Streptomyces griseorubiginosus |  |
| 1,3-phenanthroline | - |
Streptomyces griseorubiginosus | |
| AlCl3 | - |
Streptomyces griseorubiginosus | |
| BaCl2 | - |
Streptomyces griseorubiginosus | |
| citrate | - |
Streptomyces griseorubiginosus | |
| CoCl2 | - |
Streptomyces griseorubiginosus | |
| CuCl2 | - |
Streptomyces griseorubiginosus | |
| Cys | - |
Streptomyces griseorubiginosus | |
| dithiothreitol | - |
Streptomyces griseorubiginosus | |
| EDTA | - |
Streptomyces griseorubiginosus | |
| FeCl2 | - |
Streptomyces griseorubiginosus | |
| FeCl3 | - |
Streptomyces griseorubiginosus | |
| Hg2+ | HgCl2 | Streptomyces griseorubiginosus | |
| iodoacetate | - |
Streptomyces griseorubiginosus | |
| K2HPO4 | - |
Streptomyces griseorubiginosus | |
| K2SO3 | - |
Streptomyces griseorubiginosus | |
| KCl | - |
Streptomyces griseorubiginosus | |
| MnCl2 | - |
Streptomyces griseorubiginosus | |
| NaCl | - |
Streptomyces griseorubiginosus | |
| NEM | - |
Streptomyces griseorubiginosus | |
| NiCl2 | - |
Streptomyces griseorubiginosus | |
| p-(amidinophenyl)methanesulfonyl fluoride | - |
Streptomyces griseorubiginosus | |
| PCMB | - |
Streptomyces griseorubiginosus | |
| SO42- | K2SO4 | Streptomyces griseorubiginosus | |
| Zn2+ | ZnCl2 | Streptomyces griseorubiginosus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0037 | - |
4-nitrophenyl sulfate | in presence of 1 mM CaCl2 | Streptomyces griseorubiginosus | |
| 0.017 | - |
4-nitrophenyl sulfate | in absence CaCl2 | Streptomyces griseorubiginosus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | required | Streptomyces griseorubiginosus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces griseorubiginosus | - |
arylsulfatase Es-1 | - |
| Streptomyces griseorubiginosus S980-14 | - |
arylsulfatase Es-1 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| arylsulfatase Es-1 | Streptomyces griseorubiginosus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.14 | - |
- |
Streptomyces griseorubiginosus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2'',3''-di-O-acetyletoposide 4'-sulfate + H2O | - |
Streptomyces griseorubiginosus | 2'',3''-di-O-acetyletoposide + sulfate | - |
? | |
| 4-acetylphenyl sulfate + H2O | - |
Streptomyces griseorubiginosus | 4-acetylphenol + sulfate | - |
? | |
| 4-acetylphenyl sulfate + H2O | - |
Streptomyces griseorubiginosus S980-14 | 4-acetylphenol + sulfate | - |
? | |
| 4-methylumbelliferyl sulfate + H2O | - |
Streptomyces griseorubiginosus | 4-methylumbelliferone + sulfate | - |
? | |
| 4-methylumbelliferyl sulfate + H2O | - |
Streptomyces griseorubiginosus S980-14 | 4-methylumbelliferone + sulfate | - |
? | |
| 4-nitrocatechol sulfate + H2O | - |
Streptomyces griseorubiginosus | 4-nitrocatechol + sulfate | - |
? | |
| 4-nitrocatechol sulfate + H2O | - |
Streptomyces griseorubiginosus S980-14 | 4-nitrocatechol + sulfate | - |
? | |
| 4-nitrophenyl sulfate + H2O | - |
Streptomyces griseorubiginosus | 4-nitrophenol + sulfate | - |
? | |
| 4-nitrophenyl sulfate + H2O | - |
Streptomyces griseorubiginosus S980-14 | 4-nitrophenol + sulfate | - |
? | |
| androsterone sulfate + H2O | - |
Streptomyces griseorubiginosus | androsterone + sulfate | - |
? | |
| chondroitin sulfate C + H2O | - |
Streptomyces griseorubiginosus | chondroitin + sulfate | - |
? | |
| estrone 3-sulfate + H2O | - |
Streptomyces griseorubiginosus | sulfate + estrone | - |
? | |
| estrone 3-sulfate + H2O | - |
Streptomyces griseorubiginosus S980-14 | sulfate + estrone | - |
? | |
| etoposide 4'-sulfate + H2O | - |
Streptomyces griseorubiginosus | etoposide + sulfate | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Streptomyces griseorubiginosus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 4 | 50 | 4°C: about 40% of maximal activity, 50°C: about 45% of maximal activity | Streptomyces griseorubiginosus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
30 min, stable below | Streptomyces griseorubiginosus |
| 50 | - |
30 min, about 50% loss of activity | Streptomyces griseorubiginosus |
| 70 | - |
30 min, complete loss of activity | Streptomyces griseorubiginosus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
- |
Streptomyces griseorubiginosus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | 10 | pH 7.4: about 35% of maximal activity, 10.0: about 50% of maximal activity | Streptomyces griseorubiginosus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 9.5 | 37°C, 30 min, most stable | Streptomyces griseorubiginosus |
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