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Literature summary for 3.1.6.1 extracted from
- Characterization of arylsulfatase isoenzymes from Pseudomonas aeruginosa (1972), Can. J. Microbiol., 18, 561-568.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 4-nitrophenyl sulfate | substrate inhibition | Pseudomonas aeruginosa |  |
| CN- | 20 mM NaCN | Pseudomonas aeruginosa | |
| PO43- | no inhibition | Pseudomonas aeruginosa | |
| SO42- | no inhibition | Pseudomonas aeruginosa | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0294 | - |
4-nitrophenyl sulfate | alpha enzyme | Pseudomonas aeruginosa | |
| 0.075 | - |
4-nitrophenyl sulfate | beta enzyme | Pseudomonas aeruginosa | |
| 0.166 | - |
4-nitrocatechol sulfate | beta enzyme | Pseudomonas aeruginosa | |
| 0.204 | - |
4-nitrocatechol sulfate | alpha enzyme | Pseudomonas aeruginosa | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| phosphate | activates hydrolysis of 4-nitrophenyl sulfate with the beta enzyme | Pseudomonas aeruginosa | |
| sulfate | activates hydrolysis of 4-nitrophenyl sulfate with the beta enzyme | Pseudomonas aeruginosa | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas aeruginosa | - |
two electrophoretically distinct enzyme forms: alpha and beta | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrocatechol sulfate + H2O | - |
Pseudomonas aeruginosa | 4-nitrocatechol + sulfate | - |
? | |
| 4-nitrophenyl sulfate + H2O | - |
Pseudomonas aeruginosa | 4-nitrophenol + sulfate | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 40 | 5 min, in presence of substrate, stable | Pseudomonas aeruginosa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.4 | - |
hydrolysis of 4-nitrophenyl sulfate, beta enzyme | Pseudomonas aeruginosa |
| 8.4 | - |
hydrolysis of nitrocatechol sulfate, beta enzyme | Pseudomonas aeruginosa |
| 8.8 | - |
hydrolysis of 4-nitrocatechol sulfate, alpha enzyme | Pseudomonas aeruginosa |
| 9 | - |
hydrolysis of 4-nitrophenyl sulfate, alpha enzyme | Pseudomonas aeruginosa |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 10 | stable | Pseudomonas aeruginosa |
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