General Stability | Organism |
---|---|
more stable at higher protein concentrations | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.002 | 0.005 | dGTP | - |
Escherichia coli | |
0.15 | - |
GTP | - |
Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dGTP + H2O | Escherichia coli | - |
deoxyguanosine + triphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
mutant optA1 | - |
Purification (Comment) | Organism |
---|---|
16000 fold to homogeneity | Escherichia coli |
high affinity for DNA | Escherichia coli |
Storage Stability | Organism |
---|---|
0 to -20°C, 0.1 mg/ml protein concentration, 30 days, 20% and 60% loss of activity | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dGTP + H2O | - |
Escherichia coli | deoxyguanosine + triphosphate | - |
? | |
dGTP + H2O | highly specific for | Escherichia coli | deoxyguanosine + triphosphate | - |
? | |
dGTP + H2O | no activity on dGDP | Escherichia coli | deoxyguanosine + triphosphate | - |
? | |
GTP + H2O | - |
Escherichia coli | guanosine + triphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
More | 50 fold increased level | Escherichia coli |
More | C-T transition 74 bases upstream | Escherichia coli |
More | OPTA1 mutants | Escherichia coli |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
- |
- |
reversible loss of activity at low protein concentrations | Escherichia coli |
55 | 60 | full activity after 1 h | Escherichia coli |
65 | 70 | 90% denatured after 1 h | Escherichia coli |