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Literature summary for 3.1.5.1 extracted from

  • Kornberg, S.R.; Lehman, I.R.; Bessman, M.J.; Simms, E.S.; Kornberg, A.
    Enzymatic cleavage of deoxyguanosine triphosphate to deoxyguanosine and tripolyphosphate (1957), J. Biol. Chem., 233, 159-162.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
dGTP competitive inhibitor Escherichia coli
GTP competitive inhibitor Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.002 0.005 dGTP
-
Escherichia coli
0.15
-
GTP
-
Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ no activity in absence of Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dGTP + H2O Escherichia coli
-
deoxyguanosine + triphosphate
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Escherichia coli

Storage Stability

Storage Stability Organism
stable at -20°C Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dGTP + H2O
-
Escherichia coli deoxyguanosine + triphosphate
-
?
dGTP + H2O highly specific for Escherichia coli deoxyguanosine + triphosphate
-
?
dGTP + H2O no activity on dGDP Escherichia coli deoxyguanosine + triphosphate
-
?
GTP + H2O at a lower rate than dGTP Escherichia coli guanosine + triphosphate
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8.5 70% activity at pH 9.2 Escherichia coli