Crystallization (Comment) | Organism |
---|---|
complexed with orthovanadate to 1.5 A resolution. Orthovanadate binds in a tetrahedral geometry by the two catalytic manganese ions and the putative general acid residue H200 | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
D164A | 0.07% of wild-type activity | Escherichia coli |
D187A | 83% of wild-type activity | Escherichia coli |
D54A | 0.09% of wild-type activity | Escherichia coli |
D80A | 0.07% of wild-type activity | Escherichia coli |
H200A | 10% of wild-type activity, 6.33fold decrease in kcat/KM with substituted methyl phenylphosphate diesters with leaving group pKa values ranging from 4 to 8.4. With bis(pnitrophenyl)phosphate as a substrate, there is a 10fold decrease in kcat/KM, primarily the result of a large increase in KM. The nickel ion-activated H200A mutant displays a bell-shaped pH dependence for kcat/KM with pKa values comparable to those of the wild-type enzyme | Escherichia coli |
H222A | 14% of wild-type activity | Escherichia coli |
H78A | 0.3% of wild-type activity | Escherichia coli |
T75A | 3% of wild-type activity | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
orthovanadate | crystallization data | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.4 | - |
bis(p-nitrophenyl)phosphate | mutant D54A, pH 7.1, 25°C, presence of Mn2+ | Escherichia coli | |
3 | - |
bis(p-nitrophenyl)phosphate | mutant H222A, pH 7.1, 25°C, presence of Mn2+ | Escherichia coli | |
3.4 | - |
bis(p-nitrophenyl)phosphate | mutant D187A, pH 7.1, 25°C, presence of Mn2+ | Escherichia coli | |
3.5 | - |
bis(p-nitrophenyl)phosphate | wild-type, pH 7.1, 25°C, presence of Mn2+ | Escherichia coli | |
3.7 | - |
bis(p-nitrophenyl)phosphate | mutant D164A, pH 7.1, 25°C, presence of Mn2+ | Escherichia coli | |
3.7 | - |
bis(p-nitrophenyl)phosphate | mutant D80A, pH 7.1, 25°C, presence of Mn2+ | Escherichia coli | |
3.8 | - |
bis(p-nitrophenyl)phosphate | mutant H78A, pH 7.1, 25°C, presence of Mn2+ | Escherichia coli | |
18 | - |
bis(p-nitrophenyl)phosphate | mutant H200A, pH 7.1, 25°C, presence of Mn2+ | Escherichia coli | |
28 | - |
bis(p-nitrophenyl)phosphate | mutant T75A, pH 7.1, 25°C, presence of Mn2+ | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | inhibitor orthovanadate binds in a tetrahedral geometry by the two catalytic manganese ions and the putative general acid residue H200 | Escherichia coli | |
Ni2+ | may substitute for Mn2+ | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P16692 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
bis(p-nitrophenyl)phosphate + H2O | - |
Escherichia coli | ? | - |
? | |
methyl 2,4-dichlorophenyl phosphate + H2O | - |
Escherichia coli | ? | - |
? | |
methyl 2,4-dinitrophenyl phosphate + H2O | - |
Escherichia coli | ? | - |
? | |
methyl 2,5-dinitrophenyl phosphate + H2O | - |
Escherichia coli | ? | - |
? | |
methyl 2-chloro-4-nitrophenyl phosphate + H2O | - |
Escherichia coli | ? | - |
? | |
methyl 3-nitrophenyl phosphate + H2O | - |
Escherichia coli | ? | - |
? | |
methyl 4-chlorophenyl phosphate + H2O | - |
Escherichia coli | ? | - |
? | |
methyl 4-cyanophenyl phosphate + H2O | - |
Escherichia coli | ? | - |
? | |
methyl 4-nitrophenyl phosphate + H2O | - |
Escherichia coli | ? | - |
? | |
methyl nitrophenyl phosphate + H2O | - |
Escherichia coli | ? | - |
? | |
methyl phenyl phosphate + H2O | - |
Escherichia coli | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
phnP | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
bis(p-nitrophenyl)phosphate | mutant D164A, pH 7.1, 25°C, presence of Mn2+ | Escherichia coli | |
0.001 | - |
bis(p-nitrophenyl)phosphate | mutant D54A, pH 7.1, 25°C, presence of Mn2+ | Escherichia coli | |
0.001 | - |
bis(p-nitrophenyl)phosphate | mutant D80A, pH 7.1, 25°C, presence of Mn2+ | Escherichia coli | |
0.003 | - |
bis(p-nitrophenyl)phosphate | mutant H78A, pH 7.1, 25°C, presence of Mn2+ | Escherichia coli | |
0.13 | - |
bis(p-nitrophenyl)phosphate | mutant H222A, pH 7.1, 25°C, presence of Mn2+ | Escherichia coli | |
0.25 | - |
bis(p-nitrophenyl)phosphate | mutant T75A, pH 7.1, 25°C, presence of Mn2+ | Escherichia coli | |
0.55 | - |
bis(p-nitrophenyl)phosphate | mutant H200A, pH 7.1, 25°C, presence of Mn2+ | Escherichia coli | |
0.88 | - |
bis(p-nitrophenyl)phosphate | mutant D187A, pH 7.1, 25°C, presence of Mn2+ | Escherichia coli | |
1.07 | - |
bis(p-nitrophenyl)phosphate | wild-type, pH 7.1, 25°C, presence of Mn2+ | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Escherichia coli |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
bis(p-nitrophenyl)phosphate | mutant H78A, pH 7.1, 25°C, presence of Mn2+ | Escherichia coli | |
0.0024 | - |
methyl 4-chlorophenyl phosphate | wild-type, pH 7.1, 25°C, presence of Mn2+ | Escherichia coli | |
0.006 | - |
methyl 4-cyanophenyl phosphate | wild-type, pH 7.1, 25°C, presence of Mn2+ | Escherichia coli | |
0.009 | - |
bis(p-nitrophenyl)phosphate | mutant T75A, pH 7.1, 25°C, presence of Mn2+ | Escherichia coli | |
0.009 | - |
methyl phenyl phosphate | wild-type, pH 7.1, 25°C, presence of Mn2+ | Escherichia coli | |
0.03 | - |
bis(p-nitrophenyl)phosphate | mutant H200A, pH 7.1, 25°C, presence of Mn2+ | Escherichia coli | |
0.041 | - |
methyl 3-nitrophenyl phosphate | wild-type, pH 7.1, 25°C, presence of Mn2+ | Escherichia coli | |
0.043 | - |
bis(p-nitrophenyl)phosphate | mutant H222A, pH 7.1, 25°C, presence of Mn2+ | Escherichia coli | |
0.047 | - |
methyl 4-nitrophenyl phosphate | wild-type, pH 7.1, 25°C, presence of Mn2+ | Escherichia coli | |
0.124 | - |
methyl 2,4-dichlorophenyl phosphate | wild-type, pH 7.1, 25°C, presence of Mn2+ | Escherichia coli | |
0.22 | - |
methyl 2,4-dinitrophenyl phosphate | wild-type, pH 7.1, 25°C, presence of Mn2+ | Escherichia coli | |
0.25 | - |
bis(p-nitrophenyl)phosphate | mutant D187A, pH 7.1, 25°C, presence of Mn2+ | Escherichia coli | |
0.29 | - |
methyl 2-chloro-4-nitrophenyl phosphate | wild-type, pH 7.1, 25°C, presence of Mn2+ | Escherichia coli | |
0.3 | - |
bis(p-nitrophenyl)phosphate | wild-type, pH 7.1, 25°C, presence of Mn2+ | Escherichia coli | |
0.68 | - |
methyl 2,5-dinitrophenyl phosphate | wild-type, pH 7.1, 25°C, presence of Mn2+ | Escherichia coli |