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Literature summary for 3.1.4.53 extracted from

  • Shepherd, M.; McSorley, T.; Olsen, A.E.; Johnston, L.A.; Thomson, N.C.; Baillie, G.S.; Houslay, M.D.; Bolger, G.B.
    Molecular cloning and subcellular distribution of the novel PDE4B4 cAMP-specific phosphodiesterase isoform (2003), Biochem. J., 370, 429-438.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.4.53

Cloned(Commentary)

Cloned (Comment) Organism
expression of PDE4B4 cDNA in COS7 cells. Treatment of COS7 cells with forskolin, to elevate cAMP levels, produced activation of PDE4B4, which is associated with the phosphorylation of PDE4B4 on Ser56 within UCR1 Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
rolipram i.e. 4-[3-(cyclopentoxyl)-4-methoxyphenyl]-2-pyrrolidinone Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0054
-
 adenosine 3',5'-cyclic phosphate
-
 Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Rattus norvegicus 5829
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
adenosine 3',5'-cyclic phosphate + H2O Rattus norvegicus PDE4B4 isoform may have a distinct functional role in regulating cAMP levels in specific cell types adenosine 5'-phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus P14646
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein treatment of COS7 cells with forskolin, to elevate cAMP levels, produced activation of PDE4B4, which is associated with the phosphorylation of PDE4B4 on Ser-56 within UCR1 Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
 Rattus norvegicus
-
liver
-
 Rattus norvegicus
-
skeletal muscle
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
adenosine 3',5'-cyclic phosphate + H2O
-
 Rattus norvegicus adenosine 5'-phosphate
-
 ?
adenosine 3',5'-cyclic phosphate + H2O PDE4B4 isoform may have a distinct functional role in regulating cAMP levels in specific cell types Rattus norvegicus adenosine 5'-phosphate
-
 ?

Synonyms

Synonyms Comment Organism
PDE4B4
-
 Rattus norvegicus

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.000083
-
-
 Rattus norvegicus rolipram