Literature summary for 3.1.4.53 extracted from

Alonso, G.D.; Schoijet, A.C.; Torres, H.N.; Flawia, M.M.
TcrPDEA1, a cAMP-specific phosphodiesterase with atypical pharmacological properties from Trypanosoma cruzi (2007), Mol. Biochem. Parasitol., 152, 72-79.

Search for more literature for 3.1.4.53

Activating Compound

Activating Compound	Comment	Organism	Structure
additional information	no effector: Ca2+, calmodulin	Trypanosoma cruzi

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Saccharomyces cerevisiae	Trypanosoma cruzi

Protein Variants

Protein Variants	Comment	Organism
additional information	isoform PDEA1 is able to complement a yeast strain deficient in phosphodiesterase genes	Trypanosoma cruzi

General Stability

General Stability	Organism
Ca2+ required for stability	Trypanosoma cruzi

Inhibitors

Inhibitors	Comment	Organism	Structure
additional information	not inhibitory: 3-isobutyl-1-methylxanthine, papaverine, theophylline; TcrPDEA1 is resistant to the typical phosphodiesterase inhibitors, such as IBMX, papaverine and theophylline	Trypanosoma cruzi

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.191	-	adenosine 3',5'-cyclic phosphate	-	Trypanosoma cruzi
0.191	-	cAMP	pH 7.5, 30°C	Trypanosoma cruzi

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required	Trypanosoma cruzi
Mg2+	required for activity and stability	Trypanosoma cruzi
additional information	no effector: Ca2+, calmodulin	Trypanosoma cruzi

Organism

Organism	UniProt	Comment	Textmining
Trypanosoma cruzi	Q309F4	-	-
Trypanosoma cruzi	Q309F4	isoform PDEA1	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
adenosine 3',5'-cyclic phosphate + H2O	no activity with cGMP	Trypanosoma cruzi	adenosine 5'-phosphate	-	?
cAMP + H2O	-	Trypanosoma cruzi	5'-AMP	-	?
additional information	cGMP neither activates the enzyme nor competes as a substrate	Trypanosoma cruzi	?	-	?

Synonyms

Synonyms	Comment	Organism
cAMP-specific phosphodiesterase	-	Trypanosoma cruzi
TcrPDEA1	-	Trypanosoma cruzi

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Release 2023.1 (January 2023)