Activating Compound | Comment | Organism | Structure |
---|---|---|---|
cAMP | binds to N-terminus and GAF domain, affinity of 0.0005 mM | Trypanosoma cruzi | |
cAMP | binds to N-terminus and GAF domain, affinity of 0.001 mM | Trypanosoma cruzi | |
cGMP | binds to N-terminus and GAF domain, affinity of 0.003 mM | Trypanosoma cruzi | |
additional information | enzyme does not bind cGMP | Trypanosoma cruzi |
Cloned (Comment) | Organism |
---|---|
expression of TcrPDEB1 catalytic domain in Escherichia coli | Trypanosoma cruzi |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
dipyridamole | - |
Trypanosoma cruzi | |
EHNA | - |
Trypanosoma cruzi | |
erythro-9-(2-hydroxy-3-nonyl)adenine | i.e. EHNA | Trypanosoma cruzi | |
N-[(5S)-5-[2-(3-fluorophenyl)acetamido]-6-[4-(6-methylpyridin-2-yl)piperazin-1-yl]-6-oxohexyl]prop-2-enamide | - |
Trypanosoma cruzi |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0112 | - |
adenosine 3',5'-cyclic phosphate | pH 7.5, 30°C | Trypanosoma cruzi |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
102500 | - |
x * 102500, calculated | Trypanosoma cruzi |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma cruzi | - |
isoform PDEB2, expression in all developmental stages | - |
Trypanosoma cruzi | Q49UB9 | - |
- |
Trypanosoma cruzi | Q49UB9 | isoform PDEB1, expression in all developmental stages | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2'-O-(N-methylanthraniloyl)adenosine-3',5'-cyclic monophosphate + H2O | fluorescent cAMP analogue | Trypanosoma cruzi | 2'-O-(N-methylanthraniloyl)adenosine-5'-monophosphate | - |
? | |
adenosine 3',5'-cyclic phosphate + H2O | - |
Trypanosoma cruzi | adenosine 5'-phosphate | - |
? | |
cAMP + H2O | - |
Trypanosoma cruzi | 5'-AMP | - |
? | |
additional information | no activity with up to 0.1 mM cGMP | Trypanosoma cruzi | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 102500, calculated | Trypanosoma cruzi |
More | enzyme contains two GAF domains and a catalytic domain highly homologous with that of the Trypanosoma brucei family | Trypanosoma cruzi |
Synonyms | Comment | Organism |
---|---|---|
TcrPDEB1 | - |
Trypanosoma cruzi |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.00113 | - |
pH 7.5, 30°C | Trypanosoma cruzi | dipyridamole | |
0.0113 | - |
30°C, pH 7.5 | Trypanosoma cruzi | dipyridamole | |
0.015 | - |
30°C, pH 7.5 | Trypanosoma cruzi | dipyridamole | |
0.105 | - |
30°C, pH 7.5 | Trypanosoma cruzi | N-[(5S)-5-[2-(3-fluorophenyl)acetamido]-6-[4-(6-methylpyridin-2-yl)piperazin-1-yl]-6-oxohexyl]prop-2-enamide | |
0.128 | - |
30°C, pH 7.5 | Trypanosoma cruzi | EHNA |