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Literature summary for 3.1.4.53 extracted from

  • Bader, S.; Kortholt, A.; Snippe, H.; Van Haastert, P.J.
    DdPDE4, a novel cAMP-specific phosphodiesterase at the surface of dictyostelium cells (2006), J. Biol. Chem., 281, 20018-20026.
    View publication on PubMed
Search for more literature for 3.1.4.53

Cloned(Commentary)

Cloned (Comment) Organism
the DdPDE4 catalytic domain (amino acids 264-771) is expressed in vegetative AX2 cells Dictyostelium discoideum

Protein Variants

Protein Variants Comment Organism
additional information enzyme knock-out strains show normal aggregation but impaired development from the mound stage on Dictyostelium discoideum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0096
-
 adenosine 3',5'-cyclic phosphate
-
 Dictyostelium discoideum
0.01
-
 cAMP pH 7.0, 37°C Dictyostelium discoideum
0.01
-
 3',5'-cAMP 37°C, pH 7.0 Dictyostelium discoideum

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Dictyostelium discoideum 16020
-
membrane membrane-bound enzyme with an extracellular domain Dictyostelium discoideum 16020
-
membrane DdPDE4 is a unique membrane-bound phosphodiesterase with an extracellular catalytic domain regulating intercellular cAMP during multicellular development Dictyostelium discoideum 16020
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3',5'-cAMP + H2O Dictyostelium discoideum DdPDE4 regulates intercellular cAMP during multicellular development 5'-AMP
-
 ?
adenosine 3',5'-cyclic phosphate + H2O Dictyostelium discoideum DdPDE4 is a unique membrane-bound phosphodiesterase with an extracellular catalytic domain regulating intercellular cAMP during multicellular development adenosine 5'-phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Dictyostelium discoideum
-
-
-
Dictyostelium discoideum Q86H13
-
-
Dictyostelium discoideum Q86H13 isoform PDE4, enzyme is expressed during single cell stages and expression increases after 9 h of starvation
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification protein sequence predicts a leader sequence, two transmembrane segments, and an extracellular catalytic domain Dictyostelium discoideum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3',5'-cAMP + H2O
-
 Dictyostelium discoideum 5'-AMP
-
 ?
3',5'-cAMP + H2O DdPDE4 regulates intercellular cAMP during multicellular development Dictyostelium discoideum 5'-AMP
-
 ?
adenosine 3',5'-cyclic phosphate + H2O
-
 Dictyostelium discoideum adenosine 5'-phosphate
-
 ?
adenosine 3',5'-cyclic phosphate + H2O DdPDE4 is a unique membrane-bound phosphodiesterase with an extracellular catalytic domain regulating intercellular cAMP during multicellular development Dictyostelium discoideum adenosine 5'-phosphate
-
 ?
additional information cGMP is hydrolyzed at least 100fold more slowly than cAMP Dictyostelium discoideum ?
-
 ?

Synonyms

Synonyms Comment Organism
DdPDE4
-
 Dictyostelium discoideum