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Literature summary for 3.1.4.53 extracted from
- TbPDE1, a novel class I phosphodiesterase of Trypanosoma brucei (2004), Eur. J. Biochem., 271, 637-647.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| TbPDE1 is expressed in Saccharomyces cerevisiae at levels that are sufficient to produce a clear phenotype (heat-shock resistance, growth as a smooth suspension), but that are too low to be detectable in PDE assay of cell lysates. The N-terminally truncated construct pET-PDE1-(Arg189-Thr620) is expressed in Escherichia coli as an active enzyme | Trypanosoma brucei |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| dipyridamole | IC50: 0.013 mM | Trypanosoma brucei |  |
| etazolate | IC50: 0.025 mM | Trypanosoma brucei | |
| ethaverine | IC50: 0.008 mM | Trypanosoma brucei | |
| papaverine | IC50: 0.03 mM | Trypanosoma brucei | |
| rolipram | IC50: 0.28 mM | Trypanosoma brucei | |
| sildenafil | IC50: 0.001 mM | Trypanosoma brucei | |
| trequinsin | IC50: 0.0025 mM | Trypanosoma brucei | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Trypanosoma brucei | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| bloodstream form | - |
Trypanosoma brucei | - |
| procyclic form | - |
Trypanosoma brucei | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3',5'-cAMP + H2O | specific for cAMP | Trypanosoma brucei | 5'-AMP | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| TbPDE1 | - |
Trypanosoma brucei |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.001 | - |
IC50: 0.001 mM | Trypanosoma brucei | sildenafil | |
| 0.0025 | - |
IC50: 0.0025 mM | Trypanosoma brucei | trequinsin | |
| 0.008 | - |
IC50: 0.008 mM | Trypanosoma brucei | ethaverine | |
| 0.013 | - |
IC50: 0.013 mM | Trypanosoma brucei | dipyridamole | |
| 0.025 | - |
IC50: 0.025 mM | Trypanosoma brucei | etazolate | |
| 0.03 | - |
IC50: 0.03 mM | Trypanosoma brucei | papaverine | |
| 0.28 | - |
IC50: 0.28 mM | Trypanosoma brucei | rolipram | |
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Release 2023.1 (January 2023)
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