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Literature summary for 3.1.4.52 extracted from

  • Sultan, S.Z.; Pitzer, J.E.; Boquoi, T.; Hobbs, G.; Miller, M.R.; Motaleb, M.A.
    Analysis of the HD-GYP domain cyclic dimeric GMP phosphodiesterase reveals a role in motility and the enzootic life cycle of Borrelia burgdorferi (2011), Infect. Immun., 79, 3273-3283.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.4.52

Protein Variants

Protein Variants Comment Organism
additional information construction of a pdeA pdeB double mutant and a pdeB single mutant. pdeB single mutant cells exhibit significantly increased flexing, indicating a role for cyclic di-GMP in motility. While virulence in needle-inoculated C3H/HeN mice does not appear to be altered significantly in pdeB mutant cells, these cells exhibit a reduced ability to survive in Ixodes scapularis ticks. All phenotypes are restored when the mutant is complemented Borreliella burgdorferi

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0000029
-
 cyclic di-3',5'-guanylate pH and temperature not specified in the publication Borreliella burgdorferi

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Borreliella burgdorferi

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cyclic di-3',5'-guanylate + H2O Borreliella burgdorferi BB0374/PdeB specifically hydrolyzed cyclic di-GMP 5'-phosphoguanylyl(3'-5')guanosine
-
 ?

Organism

Organism UniProt Comment Textmining
Borreliella burgdorferi
-
 gene pdeB
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cyclic di-3',5'-guanylate + H2O
-
 Borreliella burgdorferi 5'-phosphoguanylyl(3'-5')guanosine
-
 ?
cyclic di-3',5'-guanylate + H2O BB0374/PdeB specifically hydrolyzed cyclic di-GMP Borreliella burgdorferi 5'-phosphoguanylyl(3'-5')guanosine
-
 ?

Synonyms

Synonyms Comment Organism
BB0374
-
 Borreliella burgdorferi
EAL domain phosphodiesterase
-
 Borreliella burgdorferi
HD-GYP domain cyclic di-GMP phosphodiesterases
-
 Borreliella burgdorferi
HD-GYP domain cyclic dimeric GMP phosphodiesterase
-
 Borreliella burgdorferi
PdeA
-
 Borreliella burgdorferi
PdeB
-
 Borreliella burgdorferi

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Borreliella burgdorferi

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Borreliella burgdorferi

General Information

General Information Comment Organism
physiological function PdeB is a functional cyclic di-GMP PDE, and PdeA and PdeB are the only active PDEs in Borrelia burgdorferi Borreliella burgdorferi