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Literature summary for 3.1.4.52 extracted from

  • Christen, M.; Christen, B.; Folcher, M.; Schauerte, A.; Jenal, U.
    Identification and characterization of a cyclic di-GMP-specific phosphodiesterase and its allosteric control by GTP (2005), J. Biol. Chem., 280, 30829-30837.
    View publication on PubMed
Search for more literature for 3.1.4.52

Activating Compound

Activating Compound Comment Organism Structure
GTP specifically activates phosphodiesterase activity by binding to GGDEF domain. Activation occurs via lowering the Km value for cyclic diguanylate, under physiological conditions enzyme activity is fully induced. KD value 0.004 mM Caulobacter vibrioides

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli, His-tag Caulobacter vibrioides

Protein Variants

Protein Variants Comment Organism
E323Q mutation in EAL motif, both catalytic activity and induction by GTP are not affected Caulobacter vibrioides
additional information enzyme deletion mutant shows 80% reduction in catalytic activity. Isolated C-terminal EAL domain shows no significant reduxtion in catalytic activity Caulobacter vibrioides

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ strong Caulobacter vibrioides

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00042
-
 cyclic di-3',5'-guanylate presence of 0.004 mM GTP, pH 8.0, 30°C Caulobacter vibrioides

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
 Caulobacter vibrioides
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Caulobacter vibrioides

Organism

Organism UniProt Comment Textmining
Caulobacter vibrioides
-
 protein CC3396 containing both an EAL and GGDEF motif
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.12
-
 hydrolysis of 5’-phosphoguanylyl(3’-5’)guanosine, recombinant enzyme, pH 8.0, 30°C Caulobacter vibrioides
2.42
-
 synthesis of 5’-phosphoguanylyl(3’-5’)guanosine, recombinant enzyme, pH 8.0, 30°C Caulobacter vibrioides

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5'-phosphoguanylyl(3'-5')guanosine + H2O
-
 Caulobacter vibrioides guanosine 5'-monophosphate + guanosine
-
 ?
cyclic di-3',5'-guanylate + H2O
-
 Caulobacter vibrioides 5'-phosphoguanylyl(3'-5')guanosine
-
 ?

Subunits

Subunits Comment Organism
More catalytic activity is confined to the C-terminal EAL domain. The GGDEF domain lacks diguanylate cyclase activity but is able to bind GTP which results in activation of the phosphodiresterase activity in the neighbouring EAL domain Caulobacter vibrioides

Synonyms

Synonyms Comment Organism
CC3396
-
 Caulobacter vibrioides